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	Overexpression of NAD kinase in recombinant Escherichia coli harboring the phbCAB operon improves poly(3-hydroxybutyrate) production
	Li, Zheng-Jun ; Cai, Lei ; Wu, Qiong ; Chen, Guo-Qiang
	2010-10-12 ; 2010-10-12
关键词	PHB Polyhydroxyalkanoates Cofactor engineering NAD kinase NADPH E. coli POLY-BETA-HYDROXYBUTYRATE PENTOSE-PHOSPHATE PATHWAY ALCALIGENES-EUTROPHUS H16 POLYHYDROXYALKANOIC ACIDS BIOSYNTHESIS CLONING GENE IDENTIFICATION CONSTRUCTION FERMENTATION Biotechnology & Applied Microbiology
中文摘要	NAD kinase was overexpressed to enhance the accumulation of poly(3-hydroxybutyrate) (PHB) in recombinant Escherichia coli harboring PHB synthesis pathway via an accelerated supply of NADPH, which is one of the most crucial factors influencing PHB production. A high copy number expression plasmid pE76 led to a stronger NAD kinase activity than that brought about by the low copy number plasmid pELRY. Overexpressing NAD kinase in recombinant E. coli was found not to have a negative effect on cell growth in the absence of PHB synthesis. Shake flask experiments demonstrated that excess NAD kinase in E. coli harboring the PHB synthesis operon could increase the accumulation of PHB to 16-35 wt.% compared with the controls; meanwhile, NADP concentration was enhanced threefold to sixfold. Although the two NAD kinase overexpression recombinants exhibited large disparity on NAD kinase activity, their influence on cell growth and PHB accumulation was not proportional. Under the same growth conditions without process optimization, the NAD kinase-overexpressing recombinant produced 14 g/L PHB compared with 7 g/L produced by the control in a 28-h fermentor study. In addition, substrate to PHB yield Y (PHB/glucose) showed an increase from 0.08 g PHB/g glucose for the control to 0.15 g PHB/g glucose for the NAD kinase-overexpressing strain, a 76% increase for the Y (PHB/glucose). These results clearly showed that the overexpression of NAD kinase could be used to enhance the PHB synthesis.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING ST, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81846]
专题	清华大学
推荐引用方式 GB/T 7714	Li, Zheng-Jun,Cai, Lei,Wu, Qiong,et al. Overexpression of NAD kinase in recombinant Escherichia coli harboring the phbCAB operon improves poly(3-hydroxybutyrate) production[J],2010, 2010.
APA	Li, Zheng-Jun,Cai, Lei,Wu, Qiong,&Chen, Guo-Qiang.(2010).Overexpression of NAD kinase in recombinant Escherichia coli harboring the phbCAB operon improves poly(3-hydroxybutyrate) production..
MLA	Li, Zheng-Jun,et al."Overexpression of NAD kinase in recombinant Escherichia coli harboring the phbCAB operon improves poly(3-hydroxybutyrate) production".(2010).

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