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	Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by recombinant Escherichia coli harboring propionyl-CoA synthase gene (prpE) or propionate permease gene (prpP)
	Liu, Xiao-Wu ; Wang, Hong-Hui ; Chen, Jing-Yu ; Li, Xiao-Tao ; Chen, Guo-Qiang
	2010-10-12 ; 2010-10-12
关键词	Polyhydroxyalkanoates PHBV Biosynthesis Fed-batch culture Fermentation Recombinant DNA POLY-BETA-HYDROXYBUTYRATE ALCALIGENES-EUTROPHUS POLYHYDROXYALKANOATES CLONING ACID POLY-(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE) POLY(HYDROXYBUTYRATE-CO-HYDROXYHEXANOATE) SPECTROSCOPY COPOLYMERS BACTERIA Biotechnology & Applied Microbiology Engineering, Chemical
中文摘要	In order to enhance 3-hydroxyvalerate (3HV) fraction in copolyesters of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), the propionate permease gene prpP or the propionyl-CoA synthase gene prpE was transformed into Escherichia coli XL10-Gold with co-expression of PHB operon (phaCAB) from Rolstonia eutropha. The recombinant E. coli strains were Cultured on mixed carbon Sources composed of glucose and propionic acid to promote PHBV accumulation. It was shown that the over-expression of prpE suppressed 3HV incorporation into PHBV copolymer, which led to reduced 3HV fraction. In contrast, the over-expression of prpP improved the 3HV content from 5.6 to 14.3 mol%, followed by an increased PHBV accumulation up to 62 wt%. The results showed that the expression of prpP stimulated the uptake and utilization of propionic acid and increased the 3HV fraction in PHBV. However, the over-expression of prpE in E coli did not affect 3HV content in PHBV. Surprisingly, co-expression of prpE and prpP did not lead to any 3HV formation. This Study showed the possibility to change the PHBV composition without overdose of propionic acid which is expensive and toxic for the cells. (C) 2008 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE SA ; LAUSANNE ; PO BOX 564, 1001 LAUSANNE, SWITZERLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81845]
专题	清华大学
推荐引用方式 GB/T 7714	Liu, Xiao-Wu,Wang, Hong-Hui,Chen, Jing-Yu,et al. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by recombinant Escherichia coli harboring propionyl-CoA synthase gene (prpE) or propionate permease gene (prpP)[J],2010, 2010.
APA	Liu, Xiao-Wu,Wang, Hong-Hui,Chen, Jing-Yu,Li, Xiao-Tao,&Chen, Guo-Qiang.(2010).Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by recombinant Escherichia coli harboring propionyl-CoA synthase gene (prpE) or propionate permease gene (prpP)..
MLA	Liu, Xiao-Wu,et al."Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by recombinant Escherichia coli harboring propionyl-CoA synthase gene (prpE) or propionate permease gene (prpP)".(2010).

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