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	Preparation of cross-linked carboxymethyl chitosan for repairing sciatic nerve injury in rats
	Wang, Gan ; Lu, Guangyuan ; Ao, Qiang ; Gong, Yandao ; Zhang, Xiufang
	2010-10-12 ; 2010-10-12
关键词	Biodegradability Carboxymethylation Chitosan Cross-linking Peripheral nerve regeneration REGENERATION DEGRADATION COLLAGEN CHITIN FILMS Biotechnology & Applied Microbiology
中文摘要	A successful nerve regeneration process was achieved with nerve repair tubes made up of 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) cross-linked carboxymethyl chitosan (CM-chitosan) with improved biodegradability. Chitosan has a very slow degradation rate, while the EDC cross-linked CM-chitosan tubes degraded to 30% of original weight during 8 weeks of incubation in lysozyme solution. In vitro cell culture indicated that the CM-chitosan films presented no cytotoxicity to Schwann cells. From in vivo studies using a 10 mm rat sciatic nerve defect model investigated by histomorphometry analysis, the average diameter of the fibers and the average thickness of myelin sheath in the CM-chitosan tubes were 3.7 +/- A 0.33 and 0.33 +/- A 0.04 mu m, respectively, which demonstrated equivalence to nerve autografts (the current "gold" standard); furthermore, the average fiber density in the CM-chitosan tubes was 20.5 x 10(3)/mm(2), which was similar to that of autografts (21 x 10(3)/mm(2)) and significantly higher than that of common chitosan tubes (15.3 x 10(3)/mm(2)).
语种	英语 ; 英语
出版者	SPRINGER ; DORDRECHT ; VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81790]
专题	清华大学
推荐引用方式 GB/T 7714	Wang, Gan,Lu, Guangyuan,Ao, Qiang,et al. Preparation of cross-linked carboxymethyl chitosan for repairing sciatic nerve injury in rats[J],2010, 2010.
APA	Wang, Gan,Lu, Guangyuan,Ao, Qiang,Gong, Yandao,&Zhang, Xiufang.(2010).Preparation of cross-linked carboxymethyl chitosan for repairing sciatic nerve injury in rats..
MLA	Wang, Gan,et al."Preparation of cross-linked carboxymethyl chitosan for repairing sciatic nerve injury in rats".(2010).

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