Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain | |
Lei Wei ; Cheng Chen ; Qi Zhao ; Chun Li ; Le Cong ; Xiaoling Xu ; Yanlin Ma ; Ming Liao ; Yuanyuan Xu ; Zihe Rao | |
2010-10-12 ; 2010-10-12 | |
关键词 | Experimental/ biochemistry catalysis crystal purification crystallisation genetics genomics lattice constants microorganisms molecular biophysics proteins space groups/ purification crystallization crystallographic analysis avian infectious bronchitis virus nsp3 ADRP domain nonstructural proteins RNA transcription genome replication adenosine diphosphate-ribose-1'-phosphatase ADP-ribose-1'-monophosphatase catalysis tRNA-splicing pathway gene segment encoding cloning Escherichia coli gene expression crystal diffraction space group unit-cell parameters asymmetric unit/ A8715D Physical chemistry of biomolecular solutions condensed states A8725F Physics of subcellular structures A8265J Heterogeneous catalysis at surfaces and other surface reactions A8715B Biomolecular structure, configuration, conformation, and active sites |
中文摘要 | Avian infectious bronchitis virus (IBV) encodes 15 nonstructural proteins (nsps) which play crucial roles in RNA transcription and genome replication. One of them, nsp3, contains an ADRP (adenosine diphosphate-ribose-1'-phosphatase) domain which was revealed in recent studies to have ADP-ribose-1'-monophos- phatase (Appr-1'-pase) activity. Appr-1'-pase catalyzes the conversion of ADP- ribose-1'-monophosphate (Appr-1'-p) to ADP-ribose in the tRNA-splicing pathway. The gene segment encoding the IBV nsp3 ADRP domain has been cloned and expressed in /i Escherichia//i coli/. The protein has been crystallized and the crystals diffracted to 1.8 A resolution. They belonged to space group P1, with unit-cell parameters a = 41.1, b = 43.2, c = 48.9 A , a = 78.0, alpha = 80.0, beta = 73.6 degrees . Each asymmetric unit contains two molecules. |
语种 | 英语 |
出版者 | Published for the International Union of Crystallography by Munksgaard International Booksellers and Publishers Ltd. ; Denmark |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/81772] |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Lei Wei,Cheng Chen,Qi Zhao,et al. Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain[J],2010, 2010. |
APA | Lei Wei.,Cheng Chen.,Qi Zhao.,Chun Li.,Le Cong.,...&Zihe Rao.(2010).Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain.. |
MLA | Lei Wei,et al."Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain".(2010). |
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