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	The Molecular Mechanism of LPS-induced CHI3L1 Expression
	Gao Lei ; Cai Guo-Ping
	2010-10-12 ; 2010-10-12
关键词	osteomyelitis LPS CHI3L1 osteoblast NECROSIS-FACTOR-ALPHA HUMAN CARTILAGE GP-39 KAPPA-B ARTICULAR CHONDROCYTES STAPHYLOCOCCUS-AUREUS PROTEIN FAMILY OSTEOMYELITIS INTERLEUKIN-1 PROMOTER Biochemistry & Molecular Biology Biophysics
中文摘要	Treatment of chronic osteomyelitis has become a difficult problem in clinical medicine due to its extended pathological process, high occurrence of complication and recurrence of disease. The major pathogenesis mechanism is Gram-negative bacteria infection, such as staphylococci. LPS is an important substance found in the cell wall of Gram-negative bacteria and administration of LPS to skeleton relevant cells in vitro could simulate the pathological characteristics of osteomyelitis patients. The results of quantitative real-time PCR and Western blot showed that CHI3L1 was up-regulated obviously in the infected bone tissues of osteomyelitis patients and LPS-stimulated osteoblasts. Analysis of the luciferase activity of NF-kappa B reporter gene vector revealed that LPS could activate NF-kappa B. Bay11-7082, an inhibitor of NF-kappa B activation, suppressed the elevation of CHI3L1 expression induced by LPS. Pre-incubation of osteoblasts with anti-TNF-alpha antibody or silencing TNF-alpha receptor expression by siRNA inhibited the induction effect of LPS on CHI3L1 Inhibition of NF-kappa B activation also prevented up-regulation of TNF-alpha induced by LPS. In conclusion, LPS stimulated TNF-alpha expression through activating NF-kappa B, then TNF-alpha induced CHI3L1 expression. It was demonstrated for the first time that CHI3L1 expression is promoted in osteomyelitis and LPS-treated osteoblasts and investigates the molecular mechanism of LPS-induced CHI3L1 expression in osteoblasts.
语种	中文 ; 中文
出版者	CHINESE ACAD SCIENCES, INST BIOPHYSICS ; BEIJING ; 15 DATUN RD, CHAOYAND DISTRICT, BEIJING, 100101, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81738]
专题	清华大学
推荐引用方式 GB/T 7714	Gao Lei,Cai Guo-Ping. The Molecular Mechanism of LPS-induced CHI3L1 Expression[J],2010, 2010.
APA	Gao Lei,&Cai Guo-Ping.(2010).The Molecular Mechanism of LPS-induced CHI3L1 Expression..
MLA	Gao Lei,et al."The Molecular Mechanism of LPS-induced CHI3L1 Expression".(2010).

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