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	Development of a chemiluminescent imaging assay for the detection of anti-erythropoietin antibody in human sera
	Wang, Wenjun ; Lu, Yanyan ; Zhang, Sichun ; Wang, Shidong ; Cao, Po ; Tian, Yaping ; Zhang, Xinrong
	2010-10-12 ; 2010-10-12
关键词	erythropoietin doping anti-erythropoietin antibodies chemiluminescent-imaging assay enzyme-linked immunosorbent assay serum samples RED-CELL APLASIA RECOMBINANT-HUMAN-ERYTHROPOIETIN SYSTEMIC-LUPUS-ERYTHEMATOSUS ANEMIA URINE AUTOANTIBODIES ELISA IMMUNOASSAY Biochemistry & Molecular Biology
中文摘要	Measuring low amounts of anti-erythropoietin antibodies (anti-EPO Abs) is important to evaluate the therapeutic safety of recombinant human erythropoietin (rhEPO). In this work, a simple, sensitive and high-throughput chemiluminescent (CL) imaging assay was developed for the detection of anti-EPO Abs in human sera. The influence of several physicochemical parameters, such as coating conditions, incubation time, detergent concentration and exposure time, were investigated. A calibration curve was established and the range of quantitative detection was 0.12-13.91 ng/mL. The limit of detection (LOD, 30;) for the CL-imaging assay was 0.033 ng/mL. Compared to conventional colorimetric enzyme-linked immunosorbent assay (ELISA), the LOD of the CL-imaging assay is 50-fold lower. The recoveries of anti-EPO Abs in the fortified serum were in the range 87.1-116.9% using the present method, which highlighted the validity of the CL-imaging assay system to accurately determine the anti-EPO Abs in serum samples. CL-imaging assay was used to evaluate the presence of anti-EPO Abs in serum samples obtained from chronic renal failure (CRF) patients treated with rhEPO. Contrary to what was expected, the sera from CRF patients did not contain anti-EPO Abs. Copyright (c) 2008 John Wiley & Sons, Ltd.
语种	英语 ; 英语
出版者	JOHN WILEY & SONS LTD ; CHICHESTER ; THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81090]
专题	清华大学
推荐引用方式 GB/T 7714	Wang, Wenjun,Lu, Yanyan,Zhang, Sichun,et al. Development of a chemiluminescent imaging assay for the detection of anti-erythropoietin antibody in human sera[J],2010, 2010.
APA	Wang, Wenjun.,Lu, Yanyan.,Zhang, Sichun.,Wang, Shidong.,Cao, Po.,...&Zhang, Xinrong.(2010).Development of a chemiluminescent imaging assay for the detection of anti-erythropoietin antibody in human sera..
MLA	Wang, Wenjun,et al."Development of a chemiluminescent imaging assay for the detection of anti-erythropoietin antibody in human sera".(2010).

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