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	Development of Magnetic Particle-based Chemiluminescence Enzyme Immunoassay for the Detection of 17 beta-estradiol in Environmental Water
	Xin, Tian-Bing ; Wang, Xu ; Jin, Hui ; Liang, Shu-Xuan ; Lin, Jin-Ming ; Li, Zhen-Jia
	2010-10-12 ; 2010-10-12
关键词	Chemiluminescence enzyme immunoassay (CLEIA) 17 beta-Estradiol (E2) Magnetic particles (MPs) Water samples TANDEM MASS-SPECTROMETRY ELECTROCHEMILUMINESCENCE ASSAY ESTRADIOL ESTROGENS SAMPLES HEALTH Biochemistry & Molecular Biology Biotechnology & Applied Microbiology
中文摘要	In the present work, a simple, fast, and highly sensitive chemiluminescence enzyme immunoassay for 17 beta-estradiol (E2) in environmental water samples was developed, using magnetic particles (MPs) labeled with secondary antibody as both the immobilization matrix and the separation tools. The specific anti-E2 polyclonal antibody (PcAb) was produced against a conjugate of estradiol-bovine serum albumin. The specificity of the anti-E2 antibody was studied. The results showed that the antibody did not cross-react with the structurally related endocrine-disrupting compounds, including estrone, ethinyl E2, estriol, E2-17-glucuronide, E2-3-sulfate-17-glucuronide, androstenedione, and dihydrotestosterone. The water samples were pretreated with solid-phase extraction using C-18 cartridges for the removal of matrix effects. Several physicochemical parameters including the dilution ratios of E2-6-horseradish peroxidase conjugate and anti-E2 PcAb, immunoreaction time, volume of chemiluminescent substrate and MPs, chemiluminescence reaction time, and pH of assay solution were studied and optimized. At optimal experimental conditions, it was found that the proposed method exhibited high performance with detection limit of 2.0 pg/mL, linear range of 20-1,200 pg/mL, and total assay time of 45 min. Both inter- and intra-assay coefficient of variation were less than 10%. The average recoveries of three different spiked concentration samples ranged from 86.3% to 108%. The method was successfully applied to the determination of E2 in river, waste, and tap water, and showed a good correlation with the commercially available radioimmunoassay kit.
语种	英语 ; 英语
出版者	HUMANA PRESS INC ; TOTOWA ; 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81026]
专题	清华大学
推荐引用方式 GB/T 7714	Xin, Tian-Bing,Wang, Xu,Jin, Hui,et al. Development of Magnetic Particle-based Chemiluminescence Enzyme Immunoassay for the Detection of 17 beta-estradiol in Environmental Water[J],2010, 2010.
APA	Xin, Tian-Bing,Wang, Xu,Jin, Hui,Liang, Shu-Xuan,Lin, Jin-Ming,&Li, Zhen-Jia.(2010).Development of Magnetic Particle-based Chemiluminescence Enzyme Immunoassay for the Detection of 17 beta-estradiol in Environmental Water..
MLA	Xin, Tian-Bing,et al."Development of Magnetic Particle-based Chemiluminescence Enzyme Immunoassay for the Detection of 17 beta-estradiol in Environmental Water".(2010).

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