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	An electrochemical biosensor for alpha-fetoprotein based on carbon paste electrode constructed of room temperature ionic liquid and gold nanoparticles
	Ding, Caifeng ; Zhao, Fei ; Ren, Rui ; Lin, Jin-Ming
	2010-10-12 ; 2010-10-12
关键词	Electrochemical immunoassay alpha-Fetoprotein (AFP) Carbon paste electrode (CPE) Gold nanoparticle Room temperature ionic liquid (RTIL) O-Aminophenol (OAP) TIME-RESOLVED FLUOROIMMUNOASSAY ENZYME-LINKED IMMUNOASSAY COLLOIDAL-GOLD CYTOCHROME-C IMMUNOSENSOR ALPHA-1-FETOPROTEIN SOLVENTS ANTIBODY SYSTEM FILM Chemistry, Analytical
中文摘要	A novel and effective electrochemical immunosensor for the rapid determination of alpha-fetoprotein (AFP) based on carbon paste electrode (CPE) consisting of room temperature ionic liquid (RTIL) N-butylpyridinium hexafluorophosphate (BPPF6) and graphite. The Surface of the CPE was modified with gold nanoparticles for the immobilization of the alpha-fetoptotein antibody (anti-AFP). By sandwiching the antigen between anti-AFP on the CPE modified with gold nanoparticles and the secondary antibody, polyclonal anti-human-AFP labeled with horseradish peroxidase (HRP-labeled anti-AFP), the immunoassay was established. The concentration of AFP was determined based on differential pulse voltammetry (DPV) signal, which was generated in the reaction between O-aminophenol (OAP) and H2O2 catalyzed by HRP labeled oil the sandwich immunosensor AFP concentration Could be measured in a linear range of 0.50-80.00 ng mL(-1) with a detection limit of 0.25 ng mL(-1). The immunosensor exhibited high sensitivity and good stability. and would be valuable for clinical assay of AFP. (c) 2009 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/80963]
专题	清华大学
推荐引用方式 GB/T 7714	Ding, Caifeng,Zhao, Fei,Ren, Rui,et al. An electrochemical biosensor for alpha-fetoprotein based on carbon paste electrode constructed of room temperature ionic liquid and gold nanoparticles[J],2010, 2010.
APA	Ding, Caifeng,Zhao, Fei,Ren, Rui,&Lin, Jin-Ming.(2010).An electrochemical biosensor for alpha-fetoprotein based on carbon paste electrode constructed of room temperature ionic liquid and gold nanoparticles..
MLA	Ding, Caifeng,et al."An electrochemical biosensor for alpha-fetoprotein based on carbon paste electrode constructed of room temperature ionic liquid and gold nanoparticles".(2010).

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