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	Chip-based enantioselective open-tubular capillary electrochromatography using bovine serum albumin-gold nanoparticle conjugates as the stationary phase
	Li, Hai-Fang ; Zeng, Hulie ; Chen, Zhifeng ; Lin, Jin-Ming
	2010-10-12 ; 2010-10-12
关键词	BSA Chiral stationary phase Gold nanoparticle Microchip Open-tubular CEC INDUCED FLUORESCENCE DETECTOR TOTAL ANALYSIS SYSTEMS MICROCHIP ELECTROPHORESIS ELECTROOSMOTIC FLOW CHIRAL SEPARATIONS POLYELECTROLYTE MULTILAYERS LIQUID-CHROMATOGRAPHY COLUMN DRUGS TECHNOLOGY Biochemical Research Methods Chemistry, Analytical
中文摘要	In this study, chip-based enantioselective open-tubular CEC (OT-CEC) was developed employing BSA-gold nanoparticle (GNP) conjugates as a chiral stationary phase. An immobilization procedure was realized by prederivatization of the glass microchannel with (3-mercaptopropyl)-trimethoxysilane to provide thiol groups, which linked the BSA-GNP conjugates on the inner surface of the microchannels. Incorporation of GNPs into immobilization of BSA selectors greatly increased the BSA phase ratio and favored the BSA stationary phase generated sufficient EOF. Good resolutions of FITC-labeled ephedrine and norephedrine isomers were achieved with 36 mm. effective separation channel length within 250 s. The constructed OT-CEC microdevice exhibited good repeatabilities for run-to-run enantioseparations and kept an enantioselective lifetime of more than 1 month. The effects of pH values and concentrations of a running buffer on the selectivity and resolution of enantioseparations were investigated.
语种	英语 ; 英语
出版者	WILEY-V C H VERLAG GMBH ; WEINHEIM ; PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/80857]
专题	清华大学
推荐引用方式 GB/T 7714	Li, Hai-Fang,Zeng, Hulie,Chen, Zhifeng,et al. Chip-based enantioselective open-tubular capillary electrochromatography using bovine serum albumin-gold nanoparticle conjugates as the stationary phase[J],2010, 2010.
APA	Li, Hai-Fang,Zeng, Hulie,Chen, Zhifeng,&Lin, Jin-Ming.(2010).Chip-based enantioselective open-tubular capillary electrochromatography using bovine serum albumin-gold nanoparticle conjugates as the stationary phase..
MLA	Li, Hai-Fang,et al."Chip-based enantioselective open-tubular capillary electrochromatography using bovine serum albumin-gold nanoparticle conjugates as the stationary phase".(2010).

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