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	Label-free detection of phage-displayed protein chips
	Liu Fangfang ; Luo Zhaofeng ; Ding Xiang ; Zhu Shenggeng ; Yu Xinglong
	2010-10-12 ; 2010-10-12
关键词	Practical/ biochemistry lab-on-a-chip molecular biophysics proteins surface plasmon resonance/ label-free detection phage-displayed protein chip surface plasmon resonance high sensitivity detection coated protein pill amine coupling method Biacore3000 antibody detection/ A8770 Biomedical engineering A8715K Biomolecular interactions, charge transfer complexes A8715D Physical chemistry of biomolecular solutions condensed states B7510 Biomedical measurement and imaging
中文摘要	Surface plasmon resonance and the phage display technique were combined to enable use of a variety of specific reagents for label-free, high sensitivity detection in protein chips. Phage M13 which displays 12 peptides on its coated protein pill was selected as the probe to prepare the phage-displayed protein chip using the amine coupling method. The specific interactions between the chip and the antibodies were detected using Biacore3000, with the binding kinetic parameters determined. Tests show that an antibody concentration of 0. 4 /i mu /mol ldr L/sup -1/ gives response units of 365 +/- 8, and that the relationship between the binding responses and the various antibody concentrations is well fit by the sigmoid curve. The method can be used to optimize the detecting conditions for phage-displayed protein chips.
语种	中文
出版者	Tsinghua University Press ; China
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/80217]
专题	清华大学
推荐引用方式 GB/T 7714	Liu Fangfang,Luo Zhaofeng,Ding Xiang,et al. Label-free detection of phage-displayed protein chips[J],2010, 2010.
APA	Liu Fangfang,Luo Zhaofeng,Ding Xiang,Zhu Shenggeng,&Yu Xinglong.(2010).Label-free detection of phage-displayed protein chips..
MLA	Liu Fangfang,et al."Label-free detection of phage-displayed protein chips".(2010).

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