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	Preparation and evaluation of monoclonal antibody against nitroaniline
	Peng Fangyi ; He Miao ; Song Baodong ; Jiang Hairong ; Sheng Jianwu ; Shi Hanchang
	2010-10-12 ; 2010-10-12
关键词	Experimental/ environmental factors/ monoclonal antibody nitroaniline residuals enzyme linked immuno-sorbent assay reagent kit bovine serum albumin hybridoma cell lines routine hybridoma technology ascite titer nitroaniline residues environmental monitoring/ E0230 Environmental issues
中文摘要	A monoclonal antibody (McAb) against nitroaniline was prepared for measuring nitroaniline residuals and for an enzyme linked immuno-sorbent assay (ELISA) reagent kit. Balb/c mice were immunized intraperitoneally with nitroaniline coupled with bovine serum albumin. Hybridoma cell lines secreting McAbs were obtained using routine hybridoma technology and ELISA screening. The McAbs subtype was IgG/sub 1/. The ascite titer was 10/sup -7/. The McAbs had no cross-reaction with some analogues of nitroaniline. The results show that the indirect competitive ELISA can be used to accurately detect nitroaniline residues in various water samples and be benefit for environmental monitoring.
语种	中文
出版者	Tsinghua University Press ; China
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79791]
专题	清华大学
推荐引用方式 GB/T 7714	Peng Fangyi,He Miao,Song Baodong,et al. Preparation and evaluation of monoclonal antibody against nitroaniline[J],2010, 2010.
APA	Peng Fangyi,He Miao,Song Baodong,Jiang Hairong,Sheng Jianwu,&Shi Hanchang.(2010).Preparation and evaluation of monoclonal antibody against nitroaniline..
MLA	Peng Fangyi,et al."Preparation and evaluation of monoclonal antibody against nitroaniline".(2010).

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