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	Expression of lignin peroxidase H2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia strain
	Wang Wei ; Wen Xianghua
	2010-10-12 ; 2010-10-12
关键词	heterologous expression lignin peroxidase Pichia pastoris WHITE-ROT FUNGI HETEROLOGOUS EXPRESSION ESTROGENIC ACTIVITY ESCHERICHIA-COLI ENZYMES REMOVAL DECOLORIZATION BACULOVIRUS CULTURE DYE Environmental Sciences
中文摘要	The lipH2 gene, encoding the expression of lignin peroxidase, was cloned from Phanerochaete chrysosporium BKM-F-1767 and expressed in Pichia pastoris X-33, a yeast. The cDNA of LiPH2 was generated from total RNA extracted from P. chrysosporium by PCR with primers that do not contain a P. chrysosporium lignin peroxidase secretion signal. The gene was then successfully inserted into the expression vector pPICZ alpha, and resulted in the recombinant vector pPICZ alpha-lipH2. The transformation was conducted in two ways. One was using the wild Pichia pastoris as the recipients, which results in the recombinant P. pastoris with single or low liPH2 gene copy. The second was using R pastoris and single or low lipH2 gene copy as the recipients, which results in the recombinant P. pastoris with multi-copies of lipH2 genes. This study firstly expressed the gene lipH2 in P. pastoris and achieved the successful expression of the lipH2 depending upon the generation of a recombinant strain that contained multiple copies. The lignin peroxidase activity reached a maximum of 15 U/L after 12 h induction.
语种	英语 ; 英语
出版者	SCIENCE PRESS ; BEIJING ; 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79790]
专题	清华大学
推荐引用方式 GB/T 7714	Wang Wei,Wen Xianghua. Expression of lignin peroxidase H2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia strain[J],2010, 2010.
APA	Wang Wei,&Wen Xianghua.(2010).Expression of lignin peroxidase H2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia strain..
MLA	Wang Wei,et al."Expression of lignin peroxidase H2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia strain".(2010).

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