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	Sensitive and rapid chemiluminescence enzyme immunoassay for microcystin-LR in water samples
	Long, F. ; Shi, H. C. ; He, M. ; Sheng, J. W. ; Wang, J. F.
	2010-10-12 ; 2010-10-12
关键词	Chemiluminescence Enzyme immunoassay Microcystin-LR Environmental analysis IMMUNOSENSOR ANTIBODIES CYANOBACTERIA EXPOSURE Chemistry, Analytical
中文摘要	A highly sensitive, specific, simple, and rapid chemiluminescence enzyme immunoassay (CLEIA) was developed for the determination of microcystin-LR (MC-LR). Several physicochemical parameters such as the chemiluminescent assay mediums, the dilution ratio of MC-LR-OVA conjugate, monoclonal antibody concentration, and peroxidase labeled antibody concentration were studied and optimized. Under optimum conditions, calibration curve obtained for MC-LR had detection limits of 0.032 +/- 0.003 mu g L-1. the 50% inhibition concentration (IC50) was 0.20 +/- 0.02 mu g L-1 and the quantitative detection range was 0.062-0.65 mu g L-1. The proposed methods was successfully applied to the monitoring of MC-LR in spiked water samples without significant effect of the matrix, and the recovery of MC-LR added to water samples at different concentrations ranged from 80% to 115% with the coefficients of variation (CVs) less than 9%. The LOD attained from the calibration curves and the results obtained for the real samples demonstrate the potential use of CLEIA as a screening tool for the analysis of MC-LR in environmental samples. (C) 2009 Published by Elsevier B.V.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79642]
专题	清华大学
推荐引用方式 GB/T 7714	Long, F.,Shi, H. C.,He, M.,et al. Sensitive and rapid chemiluminescence enzyme immunoassay for microcystin-LR in water samples[J],2010, 2010.
APA	Long, F.,Shi, H. C.,He, M.,Sheng, J. W.,&Wang, J. F..(2010).Sensitive and rapid chemiluminescence enzyme immunoassay for microcystin-LR in water samples..
MLA	Long, F.,et al."Sensitive and rapid chemiluminescence enzyme immunoassay for microcystin-LR in water samples".(2010).

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