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	Introduction of an NADH regeneration system into Klebsiella oxytoca leads to an enhanced oxidative and reductive metabolism of glycerol
	Zhang, Yanping ; Huang, Zhihua ; Du, Chenyu ; Li, Yin ; Cao, Zhu'an
	2010-10-12 ; 2010-10-12
关键词	NADH regeneration NADH availability Formate dehydrogenase Redox Dismutation metabolism network Klebsiella oxytoca ANAEROBIC CONTINUOUS-CULTURE ESCHERICHIA-COLI SACCHAROMYCES-CEREVISIAE FORMATE DEHYDROGENASE PNEUMONIAE 1,3-PROPANEDIOL FERMENTATION PATHWAY FLUXES AVAILABILITY Biotechnology & Applied Microbiology
中文摘要	Redox cofactors play crucial roles in the metabolic and regulatory network of living organisms. We reported here the effect of introducing a heterogeneous NADH regeneration system into Klebsiella oxytoca on cell growth and glycerol metabolism. Expression of fdh gene from Candida boidinii in K. oxytoca resulted in higher intracellular concentrations of both NADH and NAD(+) during the fermentation metaphase, with the ratio of NADH to NAD(+) unaltered and cell growth unaffected, interestingly different from that in engineered Escherichia coli, Lactococcus lactis, and others. Metabolic flux analysis revealed that fluxes to 1,3-propanediol, ethanol, and lactate were all increased, suggesting both the oxidative and reductive metabolisms of glycerol were enhanced. It demonstrates that in certain microbial system NADH availability can be increased with NADH to NAD(+) ratio unaltered, providing a new strategy to improve the metabolic flux in those microorganisms where glycolysis is not the only central metabolic pathways. (C) 2008 Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE ; SAN DIEGO ; 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79531]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, Yanping,Huang, Zhihua,Du, Chenyu,et al. Introduction of an NADH regeneration system into Klebsiella oxytoca leads to an enhanced oxidative and reductive metabolism of glycerol[J],2010, 2010.
APA	Zhang, Yanping,Huang, Zhihua,Du, Chenyu,Li, Yin,&Cao, Zhu'an.(2010).Introduction of an NADH regeneration system into Klebsiella oxytoca leads to an enhanced oxidative and reductive metabolism of glycerol..
MLA	Zhang, Yanping,et al."Introduction of an NADH regeneration system into Klebsiella oxytoca leads to an enhanced oxidative and reductive metabolism of glycerol".(2010).

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