QQ客服 官方微博 反馈留言

	Fabrication of a Novel Hybrid Scaffold for Tissue Engineered Heart Valve
	Hong, Hao ; Dong, Nianguo ; Shi, Jiawei ; Chen, Si ; Guo, Chao ; Hu, Ping ; Qi, Hongxu
	2010-10-12 ; 2010-10-12 ; OCT
关键词	tissue engineered heart valve hybrid scaffold electrospinning mesenchymal stem cells IN-VITRO CELLS PROLIFERATION MATRICES Biochemistry & Molecular Biology
中文摘要	The aim of this study was to fabricate biomatrix/polymer hybrid scaffolds using an electrospinning technique. Then tissue engineered heart valves were engineered by seeding mesenchymal stromal cells (MSCs) onto the scaffolds. The effects of the hybrid scaffolds on the proliferation of seed cells, formation of extracellular matrix and mechanical properties of tissue engineered heart valves were investigated. MSCs were obtained from rats. Porcine aortic heart valves were decellularized, coated with poly(3-hydroxybutyrate-co-4-hydroxybutyrate) using an electrospinning technique, and reseeded and cultured over a time period of 14 days. In control group, the decellularized valve scaffolds were reseeded and cultured over an equivalent time period. Specimens of each group were examined histologically (hematoxylin-eosin [HE] staining, immunohistostaining, and scanning electron microscopy), biochemically (DNA and 4-hydroxyproline) and mechanically. The results showed that recellularization was comparable to the specimens of hybrid scaffolds and controls. The specimens of hybrid scaffolds and controls revealed comparable amounts of cell mass and 4-hydroxyproline (P > 0.05). However, the specimens of hybrid scaffolds showed a significant increase in mechanical strength, compared to the controls (P < 0.05). This study demonstrated the superiority of the hybrid scaffolds to increase the mechanical strength of tissue engineered heart valves. And compared to the decellularized valve scaffolds, the hybrid scaffolds showed similar effects on the proliferation of MSCs and formation of extracellular matrix. It was believed that the hybrid scaffolds could be used for the construction of tissue engineered heart valves.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING ST, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79464]
专题	清华大学
推荐引用方式 GB/T 7714	Hong, Hao,Dong, Nianguo,Shi, Jiawei,et al. Fabrication of a Novel Hybrid Scaffold for Tissue Engineered Heart Valve[J],2010, 2010, OCT.
APA	Hong, Hao.,Dong, Nianguo.,Shi, Jiawei.,Chen, Si.,Guo, Chao.,...&Qi, Hongxu.(2010).Fabrication of a Novel Hybrid Scaffold for Tissue Engineered Heart Valve..
MLA	Hong, Hao,et al."Fabrication of a Novel Hybrid Scaffold for Tissue Engineered Heart Valve".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。