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	Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant
	Ma, Yuchao ; Yu, Huimin ; Pan, Wenyu ; Liu, Changchun ; Zhang, Shuliang ; Shen, Zhongyao
	2010-10-12 ; 2010-10-12
关键词	Acrylamide Nitrile hydratase Rhodococcus ruber Amidase knockout ESCHERICHIA-COLI INDUSTRIAL-PRODUCTION RHODOCHROUS J1 AMIDASE PURIFICATION EXPRESSION GENE ACRYLAMIDE CLONING CATALYST Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels
中文摘要	Microbial transformation of acrylonitrile to acrylamide by nitrile hydratase is of great interest to green chemistry. During the transformation, acrylic acid is generally accumulated as a by-product through biocatalysis of amidase. A novel strain with high nitrile hydratase activity was isolated from soil and identified as Rhodococcus ruber TH by morphology and 16S rRNA gene analysis. An amidase-negative recombinant, R. ruber TH3 was constructed. Its nitrile hydratase activity was 25% higher than that of the wild type, reaching 490 +/- 29.2 U/mg dry cell weight, while the amidase activity was 60% lower. After 6 h hydration of acrylonitrile using free cells as biocatalysts at 18 degrees C. acrylamide production by TH3 was 23% higher and the production of the by-product, acrylic acid, was 87% lower than that of the wild type. This result demonstrates that the strain TH3 could be valuable for industrial applications. (C) 2009 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCI LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79397]
专题	清华大学
推荐引用方式 GB/T 7714	Ma, Yuchao,Yu, Huimin,Pan, Wenyu,et al. Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant[J],2010, 2010.
APA	Ma, Yuchao,Yu, Huimin,Pan, Wenyu,Liu, Changchun,Zhang, Shuliang,&Shen, Zhongyao.(2010).Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant..
MLA	Ma, Yuchao,et al."Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant".(2010).

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