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	In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution
	Wu, Yue ; Wang, Yujun ; Luo, Guangsheng ; Dai, Youyuan
	2010-10-12 ; 2010-10-12
关键词	CS Nanoparticles Magnetisation Immobilization Lipase CANDIDA-RUGOSA LIPASE COVALENT IMMOBILIZATION ADSORPTION WATER OIL MICROEMULSION SYSTEM Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels
中文摘要	A new and simple method has been proposed to prepare magnetic Fe3O4-chitosan (CS) nanoparticles by cross-linking with sodium tripolyphosphate (TPP), precipitation with NaOH and oxidation with O-2 in hydrochloric acid aqueous phase containing CS and Fe(OH)(2), and these magnetic CS nanoparticles were used to immobilize lipase. The effects on the sequence of adding NaOH and TPP, the reaction temperature, and the ratio of CS/Fe(OH)(2) were studied. TEM showed that the diameter of composite nanoparticles was about 80 nm, and that the magnetic Fe3O4 nanoparticles with a diameter of 20 nm were evenly dispersed in the CS materials. Magnetic measurement revealed that the saturated magnetisation of the Fe3O4-CS nanoparticles could reach 35.54 emu/g. The adsorption capacity of lipase onto nanoparticles could reach 129 mg/g; and the maximal enzyme activity was 20.02 mu mol min(-1) mg(-1) (protein), and activity retention was as high as 55.6% at a certain loading amount. (C) 2009 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCI LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79395]
专题	清华大学
推荐引用方式 GB/T 7714	Wu, Yue,Wang, Yujun,Luo, Guangsheng,et al. In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution[J],2010, 2010.
APA	Wu, Yue,Wang, Yujun,Luo, Guangsheng,&Dai, Youyuan.(2010).In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution..
MLA	Wu, Yue,et al."In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution".(2010).

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