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	Regulation of 3-hydroxypropionaldehyde accumulation in Klebsiella pneumoniae by overexpression of dhaT and dhaD genes
	Chen, Zhen ; Liu, Hongjuan ; Liu, Dehua
	2010-10-12 ; 2010-10-12
关键词	dhaT dhaD 3-Hydroxypropionaldehyde 1,3-Propanediol Klebsiella pneumoniae 1,3-PROPANEDIOL PRODUCTION CLOSTRIDIUM-BUTYRICUM GLYCEROL FERMENTATION DISSIMILATION METABOLISM CULTURE Biotechnology & Applied Microbiology
中文摘要	3-Hydroxypropionaldehyde (3-HPA), an important intermediary metabolite of 1,3-propanediol (PDO) production, would be toxic to the cell growth and led to the abnormal cessation of the fermentation process. In this study, the dhaD gene encoding glycerol dehydrogenase (GDH) and dhaT gene encoding 1,3-propanediol oxidoreductase (PDOR) were overexpressed in Klebsiella pneumoniae ACCC 10082 to decrease the 3-HPA accumulation and increase the coenzyme NADH supply. By the construction of pTD plasmid, GDH and PDOR were both overexpressed and their enzyme activities were increased by 2.6- and 3.2-fold, respectively. The enzyme activity ratio of PDOR/GDHt (glycerol dehydratase) also was increased. On the other hand, NADH production was enhanced and the ratio of NADH/NAD(+) exceeded 1 after the inducement of IPTG for the constructed strain. The two factors enhanced the transformation of 3-HPA to PDO. in the batch and fed-batch fermentation by the constructed strain, the peak of 3-HPA accumulation reduced by 52.2% and 33.3%, respectively, compared with the control. The PDO concentration and yield reached 59.2 g/L and 0.48 mol/mol, respectively. Furthermore, the fed-batch fermentation process appeared easier to be regulated. This work is considered helpful for the further understanding on the PDO metabolic mechanism of K. pneumoniae and also useful for the PDO fermentation in a large-scale bioreactor. (C) 2009 Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE INC ; NEW YORK ; 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79366]
专题	清华大学
推荐引用方式 GB/T 7714	Chen, Zhen,Liu, Hongjuan,Liu, Dehua. Regulation of 3-hydroxypropionaldehyde accumulation in Klebsiella pneumoniae by overexpression of dhaT and dhaD genes[J],2010, 2010.
APA	Chen, Zhen,Liu, Hongjuan,&Liu, Dehua.(2010).Regulation of 3-hydroxypropionaldehyde accumulation in Klebsiella pneumoniae by overexpression of dhaT and dhaD genes..
MLA	Chen, Zhen,et al."Regulation of 3-hydroxypropionaldehyde accumulation in Klebsiella pneumoniae by overexpression of dhaT and dhaD genes".(2010).

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