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	Apoptotic effects of Tian-Long compound on endometrial adenocarcinoma cells in vitro
	Li, Zhong-Lian ; Morishima, Syoko ; Tang, Jin-Tian ; Otsuki, Yoshinori
	2010-10-12 ; 2010-10-12
关键词	Adenocarcinoma Apoptosis Endometrium Solanum lyratum Thunb Duchesnea chrysantha Curcuma aromatica Salisb Solanum nigrum L. HUMAN UTERINE ENDOMETRIUM ANGELICA-SINENSIS THERAPY DEATH INDUCTION DISEASE GROWTH CANCER Biology Microscopy Pathology
中文摘要	The Tian-Long (TL) compound is a water-soluble extract of six Chinese medicinal herbs. To explore its antitumor properties and the mechanism for activity in gynecological malignancies, the present studies were carried out using Ishikawa cells derived from uterine endometrial adenocarcinoma. Morphologically, cell death and decrease in the number of viable cells were observed in the presence of the TL compound. The proliferation of Ishikawa cells was significantly suppressed in a time- and dose-dependent manner, as indicated by both the WST-1 and the BrdU incorporation assay. Results from both the WST-1 and the BrdU incorporation assay demonstrated that the compound could inhibit the cell proliferation despite the presence of 17 beta-estradiol in the medium. It is generally noted that the disturbance in mitochondrial function and DNA synthesis during cell proliferation can result in apoptosis. Being consistent with this notion, redistribution of the plasma membrane phosphatidylserine was identified with fluoromicroscopy and flow cytometry. Analysis of the fluorescent patterns of JC-1 staining revealed depolarization of the mitochondrial membrane in the exposed cells. Moreover, the amount of Bcl-2 enhanced in the presence of 17 beta-estradiol was repressed by the compound. The present results indicate that the ingredients of TL compound are very promising for use in the treatment of endometrial adenocarcinoma. Further studies are necessary to elucidate the therapeutic mechanisms in its antitumor activity.
语种	英语 ; 英语
出版者	SPRINGER TOKYO ; TOKYO ; 1-11-11 KUDAN-KITA, CHIYODA-KU, TOKYO, 102-0073, JAPAN
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78727]
专题	清华大学
推荐引用方式 GB/T 7714	Li, Zhong-Lian,Morishima, Syoko,Tang, Jin-Tian,et al. Apoptotic effects of Tian-Long compound on endometrial adenocarcinoma cells in vitro[J],2010, 2010.
APA	Li, Zhong-Lian,Morishima, Syoko,Tang, Jin-Tian,&Otsuki, Yoshinori.(2010).Apoptotic effects of Tian-Long compound on endometrial adenocarcinoma cells in vitro..
MLA	Li, Zhong-Lian,et al."Apoptotic effects of Tian-Long compound on endometrial adenocarcinoma cells in vitro".(2010).

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