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	Purification, characterization and crystallization of pyrroline-5-carboxylate reductase from the hyperthermophilic archeon Sulfolobus Solfataricus
	Meng, Zhaohui ; Liu, Zhe ; Lou, Zhiyong ; Gong, Xiaocui ; Cao, Yi ; Bartlam, Mark ; Zhang, Keqin ; Rao, Zihe
	2010-10-12 ; 2010-10-12
关键词	P5CR Extremophile Crystallography Sulfolobus solfataricus DELTA(1)-PYRROLINE-5-CARBOXYLATE REDUCTASE DELTA-1-PYRROLINE-5-CARBOXYLATE REDUCTASE PYRROLINE 5-CARBOXYLATE HUMAN-ERYTHROCYTES ESCHERICHIA-COLI PROLINE APOPTOSIS GENE COMPLEMENTATION OXIDATION Biochemical Research Methods Biochemistry & Molecular Biology Biotechnology & Applied Microbiology
中文摘要	The gene SSO0495 (proC), which encodes pyrroline-5-carboxylate reductase (P5CR) from the thermoacidophilic archeon Sulfolobus solfataricus P2 (Ss-P5CR), was cloned and expressed. The purified recombinant enzyme catalyzes the thioproline dehydrogenase with concomitant oxidation of NAD(P)H to NAD(P)(+). This archeal enzyme has an optimal alkaline pH in this reversible reaction and is thermostable with a half-life of approximately 30 min at 80 degrees C. At pH 9.0, the reverse activation rate is nearly 3-fold higher than at pH 7.0. The homopolymer was characterized by cross-linking and size exclusion gel filtration chromatography. Ss-P5CR was crystallized by the hanging-drop vapor-diffusion method at 37 degrees C. Diffraction data were obtained to a resolution of 3.5 angstrom and were suitable for X-ray structure determination. (C) 2008 Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE ; SAN DIEGO ; 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78687]
专题	清华大学
推荐引用方式 GB/T 7714	Meng, Zhaohui,Liu, Zhe,Lou, Zhiyong,et al. Purification, characterization and crystallization of pyrroline-5-carboxylate reductase from the hyperthermophilic archeon Sulfolobus Solfataricus[J],2010, 2010.
APA	Meng, Zhaohui.,Liu, Zhe.,Lou, Zhiyong.,Gong, Xiaocui.,Cao, Yi.,...&Rao, Zihe.(2010).Purification, characterization and crystallization of pyrroline-5-carboxylate reductase from the hyperthermophilic archeon Sulfolobus Solfataricus..
MLA	Meng, Zhaohui,et al."Purification, characterization and crystallization of pyrroline-5-carboxylate reductase from the hyperthermophilic archeon Sulfolobus Solfataricus".(2010).

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