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	Crystal structure of Bacillus thuringiensis Cry8Ea1: An insecticidal toxin toxic to underground pests, the larvae of Holotrichia parallela
	Guo, Shuyuan ; Ye, Sheng ; Liu, Yanfeng ; Wei, Lei ; Xue, Jing ; Wu, Hongfu ; Song, Fuping ; Zhang, Jie ; Wu, Xiaoai ; Huang, Dafang ; Rao, Zihe
	2010-10-12 ; 2010-10-12
关键词	Bacillus thuringiensis Bioinsecticide Cry protein Crystal structure DELTA-ENDOTOXIN DOMAIN-III MEMBRANE INSERTION SPODOPTERA-EXIGUA AMINOPEPTIDASE-N MANDUCA-SEXTA ION-CHANNEL BINDING RECEPTORS PROTEINS Biochemistry & Molecular Biology Biophysics Cell Biology
中文摘要	Crystal (Cry) proteins belong to an insect toxin family encoded and expressed by a variety of Bacillus thuringiensis isolates, and are named due to their in vivo auto-crystallization abilities. To kill the infected host insects, protease-activated Cry toxins should firstly be recognized by certain membrane receptors on the surface of insect midgut epithelial cells and consequently assemble together as lethal transmembrane pores. Here we report the 2.2-angstrom crystal structure of Cry8Ea1 toxin, a Cry family member specifically toxic to the underground larvae of Holotrichia parallela. Superimposition of the domain I from Cry8Ea1 and other structurally characterized Cry toxins reveals an identical surface proline residue and a highly conserved kink of a helix, both of which have drawn comparatively little attention from previous researchers. Further structural analysis and functional studies suggest that both the proline and the helix kink might be essential in exposing a helix-helix hairpin, which is believed to be the very first step in the well-known "umbrella" model of the membrane penetration. In summary, we propose a plausible model of the initiation of Cry toxin domain I disassembly before membrane penetration and pore formation. (C) 2009 Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE ; SAN DIEGO ; 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78650]
专题	清华大学
推荐引用方式 GB/T 7714	Guo, Shuyuan,Ye, Sheng,Liu, Yanfeng,et al. Crystal structure of Bacillus thuringiensis Cry8Ea1: An insecticidal toxin toxic to underground pests, the larvae of Holotrichia parallela[J],2010, 2010.
APA	Guo, Shuyuan.,Ye, Sheng.,Liu, Yanfeng.,Wei, Lei.,Xue, Jing.,...&Rao, Zihe.(2010).Crystal structure of Bacillus thuringiensis Cry8Ea1: An insecticidal toxin toxic to underground pests, the larvae of Holotrichia parallela..
MLA	Guo, Shuyuan,et al."Crystal structure of Bacillus thuringiensis Cry8Ea1: An insecticidal toxin toxic to underground pests, the larvae of Holotrichia parallela".(2010).

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