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	Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea
	Gan, Zhongwei ; Yang, Jinkui ; Tao, Nan ; Lou, Zhiyong ; Mi, Qili ; Meng, Zhaohui ; Zhang, Ke-Qin
	2010-10-12 ; 2010-10-12
关键词	BOTRYTIS-CINEREA GLIOCLADIUM-ROSEUM BIOCONTROL PROTEASE CLONING DEBRIS BLIGHT Biochemical Research Methods Biochemistry & Molecular Biology Biophysics Crystallography
中文摘要	CrChi1 is a chitinase from the nematophagous fungus Clonostachys rosea that plays a role in the infection of nematodes. In order to resolve the crystal structure of CrChi1 and to gain a better understanding of its biological functions, recombinant CrChi1 was crystallized at 291 K using PEG 3350 and ammonium dihydrogen phosphate as precipitant and a 1.8 angstrom resolution X-ray data set was collected from a single flash-cooled crystal (100 K). The crystals belonged to space group P2(1), with unit-cell parameters a = 44.1, b= 71.7, c= 59.1 angstrom, alpha = gamma = 90, beta = 91.3 degrees. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient and solvent content were calculated to be 2.45 angstrom(3) DA(-1) and 40%, respectively. To our knowledge, this is the first structure determination study of a chitinase from a nematophagous fungus.
语种	英语 ; 英语
出版者	WILEY-BLACKWELL PUBLISHING, INC ; MALDEN ; COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78644]
专题	清华大学
推荐引用方式 GB/T 7714	Gan, Zhongwei,Yang, Jinkui,Tao, Nan,et al. Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea[J],2010, 2010.
APA	Gan, Zhongwei.,Yang, Jinkui.,Tao, Nan.,Lou, Zhiyong.,Mi, Qili.,...&Zhang, Ke-Qin.(2010).Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea..
MLA	Gan, Zhongwei,et al."Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea".(2010).

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