Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea | |
Gan, Zhongwei ; Yang, Jinkui ; Tao, Nan ; Lou, Zhiyong ; Mi, Qili ; Meng, Zhaohui ; Zhang, Ke-Qin | |
2010-10-12 ; 2010-10-12 | |
关键词 | BOTRYTIS-CINEREA GLIOCLADIUM-ROSEUM BIOCONTROL PROTEASE CLONING DEBRIS BLIGHT Biochemical Research Methods Biochemistry & Molecular Biology Biophysics Crystallography |
中文摘要 | CrChi1 is a chitinase from the nematophagous fungus Clonostachys rosea that plays a role in the infection of nematodes. In order to resolve the crystal structure of CrChi1 and to gain a better understanding of its biological functions, recombinant CrChi1 was crystallized at 291 K using PEG 3350 and ammonium dihydrogen phosphate as precipitant and a 1.8 angstrom resolution X-ray data set was collected from a single flash-cooled crystal (100 K). The crystals belonged to space group P2(1), with unit-cell parameters a = 44.1, b= 71.7, c= 59.1 angstrom, alpha = gamma = 90, beta = 91.3 degrees. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient and solvent content were calculated to be 2.45 angstrom(3) DA(-1) and 40%, respectively. To our knowledge, this is the first structure determination study of a chitinase from a nematophagous fungus. |
语种 | 英语 ; 英语 |
出版者 | WILEY-BLACKWELL PUBLISHING, INC ; MALDEN ; COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/78644] ![]() |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Gan, Zhongwei,Yang, Jinkui,Tao, Nan,et al. Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea[J],2010, 2010. |
APA | Gan, Zhongwei.,Yang, Jinkui.,Tao, Nan.,Lou, Zhiyong.,Mi, Qili.,...&Zhang, Ke-Qin.(2010).Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea.. |
MLA | Gan, Zhongwei,et al."Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea".(2010). |
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