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	Preliminary crystallographic analysis of avian infectious bronchitis virus main protease
	Jun Li ; Wei Shen ; Ming Liao ; Bartlam, M.
	2010-10-12 ; 2010-10-12
关键词	Practical Experimental/ diagnostic radiography food processing industry genetics microorganisms molecular biophysics patient diagnosis proteins X-ray crystallography X-ray diffraction/ avian infectious bronchitis virus protease genus Coronavirus chicken poultry industry gene expression gene replication proteolytic processing replicase polyproteins Escherichia coli X-ray crystallography microseeding X-ray diffraction self-rotation function/ A8715B Biomolecular structure, configuration, conformation, and active sites A8715M Interactions with radiations at the biomolecular level A8770E Patient diagnostic methods and instrumentation A8760J X-rays and particle beams (medical uses) E3602 Food industry
中文摘要	Infectious bronchitis virus (IBV) is the prototype of the genus Coronavirus. It causes a highly contagious disease which affects the respiratory, reproductive, neurological and renal systems of chickens, resulting great economic losses in the poultry industry worldwide. The coronavirus (CoV) main protease (M/sup pro/), which plays a pivotal role in viral gene expression and replication through a highly complex cascade involving the proteolytic processing of replicase polyproteins, is an attractive target for antiviral drug design. In this study, IBV M/sup pro/ was overexpressed in Escherichia coli. Crystals suitable for X-ray crystallography have been obtained using microseeding techniques and belong to space group P6/sub 1/22. X-ray diffraction data were collected in-house to 2.7 A resolution from a single crystal. The unit-cell parameters were a = b = 119.1, c = 270.7 A , alpha = beta = 90, gamma = 120 degrees . Three molecules were predicted to be present in the asymmetric unit from a calculated self-rotation function.
语种	英语
出版者	Published for the International Union of Crystallography by Munksgaard International Booksellers and Publishers Ltd. ; Denmark
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78561]
专题	清华大学
推荐引用方式 GB/T 7714	Jun Li,Wei Shen,Ming Liao,et al. Preliminary crystallographic analysis of avian infectious bronchitis virus main protease[J],2010, 2010.
APA	Jun Li,Wei Shen,Ming Liao,&Bartlam, M..(2010).Preliminary crystallographic analysis of avian infectious bronchitis virus main protease..
MLA	Jun Li,et al."Preliminary crystallographic analysis of avian infectious bronchitis virus main protease".(2010).

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