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	Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds
	Sun, Xiao-Dan ; Jeng, Lily ; Bolliet, Catherine ; Olsen, Bjorn R. ; Spector, Myron
	2010-10-12 ; 2010-10-12
关键词	Endostatin Collagen Scaffold Stem cells SMOOTH-MUSCLE ACTIN ARTICULAR-CARTILAGE TUMOR-GROWTH IN-VITRO OSTEOARTHRITIC CARTILAGE ANTITUMOR-ACTIVITY GENE DELIVERY CROSS-LINKING ANGIOGENESIS THERAPY Engineering, Biomedical Materials Science, Biomaterials
中文摘要	Angiogenesis is critical in the early stage of reparative processes and tissue regeneration, but the persistence of a vascular network may interfere with later transformation/maturation in naturally avascular tissues such as articular cartilage. Our supposition is that the timed delivery of an anti-angiogenic factor in cartilage tissue engineering may facilitate the formation of hyaline cartilage by inducing the regression of vascularization. To this end our overall goal is to prepare an off-the-shelf scaffold containing the gene for a potent anti-angiogenic factor. The objective of this study was to investigate the use of a type I/III collagen scaffold for the non-viral transfection of marrow stromal cells (MSCs, also referred to as mesenchymal stem cells) with the plasmid encoding endostatin. Caprine MSCs were transfected by the naked plasmid alone and plasmid incorporated into a cationic lipid complex in three experiments: I) cells were transfected in monolayer; 2) monolayer-transfected cells were grown in a collagen sponge-like scaffold; and 3) non-transfected cells were grown in a collagen scaffold containing the naked plasmid and endostatin lipoplex. Independent variables were the passage number of the cells and the plasmid loading. The amount of endostatin released by the cells into the medium was measured using an ELISA. The results demonstrated the overexpression of endostatin by MSCs growing in the endostatin lipoplex-supplemented collagen scaffolds. Endostatin released by the cell-seeded scaffolds reached a peak of 13 ng/ml for scaffolds incorporating as little as 20 mu g of plasmid, at the 3-day collection period ending 5 clays post-seeding. The accumulated endostatin synthesis over a 2-week period began to achieve what may be a therapeutic level. MSCs transfected with the endostatin gene in monolayer continued to express the gene when grown in the collagen scaffolds. The results demonstrate the promise of the non-viral delivery of the gene for this potent anti-angiogenic protein to MSCs via a collagen scaffold. (C) 2008 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCI LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78472]
专题	清华大学
推荐引用方式 GB/T 7714	Sun, Xiao-Dan,Jeng, Lily,Bolliet, Catherine,et al. Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds[J],2010, 2010.
APA	Sun, Xiao-Dan,Jeng, Lily,Bolliet, Catherine,Olsen, Bjorn R.,&Spector, Myron.(2010).Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds..
MLA	Sun, Xiao-Dan,et al."Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds".(2010).

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