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	Maturation of osteoblast-like SaoS2 induced by carbon nanotubes
	Li, Xiaoming ; Gao, Hong ; Uo, Motohiro ; Sato, Yoshinori ; Akasaka, Tsukasa ; Abe, Shigeaki ; Feng, Qingling ; Cui, Fuzhai ; Watari, Fumio
	2010-10-12 ; 2010-10-12
关键词	MARROW STROMAL CELLS IN-VITRO BONE-FORMATION CHITIN FIBERS STEM-CELLS SUBCUTANEOUS TISSUE NEURONAL GROWTH COLLAGEN SCAFFOLDS DIFFERENTIATION Engineering, Biomedical Materials Science, Biomaterials
中文摘要	Osteogenic maturation of the osteoblast is crucial for bone formation. In this study, multi-walled carbon nanotubes (MWCNTs) and graphite (GP) were pressed as compacts. The greater ability of carbon nanotubes to adsorb proteins, compared with graphite, was shown. Human osteoblast-like SaoS2 cells were cultured and the cell response to the two kinds of compacts was compared in vitro. Meanwhile, we used cell culture on the culture plate as a control. Assays for osteonectin, osteopontin and osteocalcin gene expression, total protein (TP) amount, alkaline phosphatase activity (ALP) and DNA of cells cultured on the samples were done. During the conventional culture, significantly higher osteonectin, osteopontin and osteocalcin gene expression level, ALP/DNA and TP/DNA on carbon nanotubes were found. To confirm the hypothesis that the larger amount of specific proteins adsorbed on the carbon nanotubes was crucial for this, the compacts were pre-soaked in culture medium having additional recombinant human bone morphogenetic protein-2 (rhBMP-2) before cell culture. Compared with GP, osteonectin, osteopontin and osteocalcin gene expression level, ALP/DNA and TP/DNA of the cells tested increased more on the MWCNTs after the compacts were pre-soaked in the culture medium with rhBMP-2. The results indicated that the carbon nanotubes might induce osteogenic maturation of the osteoblast by adsorbing more specific proteins.
语种	英语 ; 英语
出版者	IOP PUBLISHING LTD ; BRISTOL ; DIRAC HOUSE, TEMPLE BACK, BRISTOL BS1 6BE, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78137]
专题	清华大学
推荐引用方式 GB/T 7714	Li, Xiaoming,Gao, Hong,Uo, Motohiro,et al. Maturation of osteoblast-like SaoS2 induced by carbon nanotubes[J],2010, 2010.
APA	Li, Xiaoming.,Gao, Hong.,Uo, Motohiro.,Sato, Yoshinori.,Akasaka, Tsukasa.,...&Watari, Fumio.(2010).Maturation of osteoblast-like SaoS2 induced by carbon nanotubes..
MLA	Li, Xiaoming,et al."Maturation of osteoblast-like SaoS2 induced by carbon nanotubes".(2010).

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