| 当归水溶性成分HPLC指纹图谱研究 | |
| 张敏 ; 胡坪 ; 罗国安 ; 王义明 ; ZHANG Min ; HU Ping ; LUO Guo-an ; WANG Yi-ming | |
| 2010-07-19 ; 2010-07-19 | |
| 关键词 | 当归 指纹图谱 高效液相色谱 Angelica sinensis fingerprint HPLC R284.1 |
| 其他题名 | HPLC fingerprint of the water-soluble constituents in Angelica Sinensis |
| 中文摘要 | 目的:建立当归水溶性成分的HPLC指纹图谱方法。方法:采用梯度洗脱法,对当归水溶性成分进行HPLC测定,采用Phenomenex Luna C18(250 mm×4.6 mm ID,5μm)色谱柱,以水(0.1%甲酸)-乙腈作为流动相,检测波长254 nm,洗脱流速0.5 mL/min,记录时间65 min。结果:对指纹图谱方法学的精密度、重复性、稳定性考察中特征峰相对峰面积、相对保留时间的RSD均小于5%。本试验把HPLC指纹图谱结合相似度分析和聚类分析用于当归药材的质量评价,较好地表达了产地和用药部位信息。结论:本试验建立的HPLC指纹图谱方法可作为当归药材水溶性成分的质量控制手段。; AIM: To establish the HPLC fingerprint method of the water-soluble constituents in Angelica Sinensis. METHODS: A Phenomenex Luna C_18 column(250 mm×4.6 mm ID,5 μm) was utilized.Water containing 0.1% formic acid and acetonitrile were used as gradient eluents.The detection wavelength was set at 254 nm and the flow rate was 0.5 mL/min.UV spectra was recorded within 65 min. RESULTS: The RSD of relative peak areas and retention time of peaks in precision,repeatability and stability has not reached 5.0%,respectively. HPLC fingerprint,combined with similarity analysis and cluster method,are used to evaluate the quality of Angelica Sinensis,and it describes the character of regions and fractions properly. CONCLUSION: The HPLC fingerprint method is practicable as a standard method in controlling the quality of the water-soluble constituents in Angelica Sinensis.; 国家高技术研究发展计划(863计划)(课题编号2003AA2Z2021) |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/76208]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 张敏,胡坪,罗国安,等. 当归水溶性成分HPLC指纹图谱研究[J],2010, 2010. |
| APA | 张敏.,胡坪.,罗国安.,王义明.,ZHANG Min.,...&WANG Yi-ming.(2010).当归水溶性成分HPLC指纹图谱研究.. |
| MLA | 张敏,et al."当归水溶性成分HPLC指纹图谱研究".(2010). |
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