| 三七剪口指纹图谱研究及应用 | |
| 张敏 ; 胡坪 ; 罗国安 ; 王义明 ; ZHANG Min ; HU Ping ; LUO Guo-an ; WANG Yi-ming | |
| 2010-07-19 ; 2010-07-19 | |
| 关键词 | 三七剪口 高效液相色谱 指纹图谱 the rhizome of Panax notoginseng (Burk.) F. H.Chen HPLC fingerprint R284 |
| 其他题名 | Application of HPLC fingerprint to analysis of rhizome of Panax notoginseng |
| 中文摘要 | 目的建立三七剪口的HPLC指纹图谱,同时用于不同产地药材的皂苷成分定量。方法采用梯度洗脱法,对三七剪口水提物进行HPLC测定,流动相为水-乙腈,检测波长203nm;记录时间65min。结果运用梯度洗脱的方法能分离三七剪口的各类组分,并可对其中三七皂苷R1、人参皂苷Rg1、Re、Rb1和Rd进行定量。结论本实验建立的HPLC指纹图谱和测定方法可作为三七剪口有效组分的控制手段。; Objective To establish the HPLC fingerprint method of the rhizome of Panax notoginseng and set up a method for content determination of the main ginsenosides in the rhizome of P. notoginseng from various habitats. MethodsHPLC Analysis was carried on C18 column with flow phase water-acetonitrile. The detection wavelength was at 203 nm, record time was 65 min. ResultsThe components can be separated well and the ginsenosides Rg1, Re, Rb1, and Rd and notoginsenoside R1 can be determinated using this method. ConclusionThe HPLC fingerprint of the rhizome of P. notoginseng and the determination method which developed in this paper are suitable for the quality control of the rhizome of P.notoginseng.; 国家高技术研究发展计划(863计划)(2003AA2Z2021) |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/76008]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 张敏,胡坪,罗国安,等. 三七剪口指纹图谱研究及应用[J],2010, 2010. |
| APA | 张敏.,胡坪.,罗国安.,王义明.,ZHANG Min.,...&WANG Yi-ming.(2010).三七剪口指纹图谱研究及应用.. |
| MLA | 张敏,et al."三七剪口指纹图谱研究及应用".(2010). |
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