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	超旱生植物沙冬青高温胁迫下的快速叶绿素荧光动力学特征
	张谧 ; 王慧娟 ; 于长青 ; ZHANG Mi ; WANG Huijuan ; YU Changqing
	2010-06-10 ; 2010-06-10
关键词	叶绿素荧光 光合 JIP方法 OKJIP曲线 性能指数 chlorophyll fluorescence photosynthesis JIP-test OKJIP curve performance index Q945
其他题名	The examination of high temperature stress of Ammopiptanthus mongolicus by chlorophyll fluorescence induction parameters
中文摘要	沙冬青Ammopiptanthus mongolicus是温带荒漠地区唯一的常绿阔叶灌木,由于其生长的沙漠中极端高温远高于其它地区,因此研究沙冬青对高温胁迫的响应特征对于解释植物的抗逆机理具有极为重要的意义。快速叶绿素荧光动力学可以在无损情况下探知叶片光合机构的热损伤程度,JIP-test方法将测量值转化为多种具有生物学意义的参数,因而被广泛应用于高温胁迫的有关研究中。对不同环境条件下快速叶绿素荧光诱导动力学曲线的分析可以深入了解植物光合机构对环境的适应机制。本文利用高频采样叶绿素荧光仪在野外下测定了沙冬青在不同温度处理下的荧光响应情况。研究结果表明,沙冬青耐热性在40~45℃;25℃、30℃及40℃时的FV/FM在0.80以上,光合反应正常。50℃时出现高温胁迫特征:1)FV/FM降低为0.73;2)OJIP曲线变形为OKJIP曲线,K点出现在300ms左右,值为245.00;3)反应中心的活性下降,QA-的还原速率加快。这些变化是由于光合反应中的电子传递受阻引起的,而光合反应中心并没有被破坏,胁迫条件解除后,光合机构还可恢复,说明沙冬青具有极强耐热性。; Ammopiptanthus mongolicus is the only broad-leaved evergreen shrubs growing in desert area. The extremely high temperature in desert is much higher than in other regions, so the study of the response of Ammopiptanthus mongolicus on high temperature stress will help understanding the mechanism of the resilience of plant to heat stress. The chlorophyll fluorescence technique was considered as an effective tool in the context of non-destructive leaf photosynthetic apparatus of the degree of thermal, and has been widely used in relevant studies of high temperature stress. The JIP-test is a method for analyze the fluorescence transient, and also known as fast-kinetic research methods, which transformed the measured value to a serials biological parameters, and also detect the energy strength generated by PSII. The results show that the thermo stability of Ammopiptanthus mongolicus is at 40～45 ℃. heat stress characteristics occurs at 50 ℃ , at that time: 1) FV / FM decreased to 0.73; 2) the chlorophyll fluorescence OJIP curve is deformation to OKJIP curve, where appears a K point in 300 ms in the curve. the value is 245.00; 3) the activity of Reaction center declined, and QA-reduction rate increases with increasing of temperature rising. All those change is due to the block of photosynthetic electron transfer, but the photosynthetic reaction center has not been destroyed. After the stress conditions, the photosynthetic apparatus can be restored, it proved that Ammopiptanthus mongolicus has a very strong heat resistance.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/65893]
专题	清华大学
推荐引用方式 GB/T 7714	张谧,王慧娟,于长青,等. 超旱生植物沙冬青高温胁迫下的快速叶绿素荧光动力学特征[J],2010, 2010.
APA	张谧,王慧娟,于长青,ZHANG Mi,WANG Huijuan,&YU Changqing.(2010).超旱生植物沙冬青高温胁迫下的快速叶绿素荧光动力学特征..
MLA	张谧,et al."超旱生植物沙冬青高温胁迫下的快速叶绿素荧光动力学特征".(2010).

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