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	微囊藻毒素-LR多克隆抗体的制备
	盛建武 ; 何苗 ; 施汉昌 ; 钱易 ; SHENG Jian-wu ; HE Miao ; SHI Han-chang ; QIAN Yi
	2010-06-10 ; 2010-06-10
关键词	微囊藻毒素-LR 多克隆抗体 间接竞争ELISA Microcystin-LR polyclonal antibody indirect competitive ELISA X832
其他题名	Preparation of Polyclonal Antibody Against Microcystin-LR
中文摘要	通过对新西兰大白兔免疫自制的微囊藻毒素-LR(Microcystin-LR,MC-LR)完全抗原BSA-MC-LR,获得了质量较好的抗MC-LR的多克隆抗体,间接ELISA表明抗体的效价能达到1.5×105;固定包被抗原OVA-MC-LR,采用间接竞争ELISA测定水体中的微囊藻毒素,标准曲线表明对水样中MC-LR的检测下限为10ng/L,线性区间为30ng/L~3μg/L,能满足对饮用水和地表水中MC-LR的检测要求.; Good quality polyclonal antibody against Microcystin-LR(MC-LR) was obtained from the New Zealand rabbit immunized with the homemade complete antigen BSA-MC-LR.Indirect ELISA shows the titer of this antibody is more than 1.5×10~5; Indirect competitive ELISA immobilized the coating antigen OVA-MC-LR was adapted to detect MCs,the calibration curve shows that the detection lower limit is 10ng/L,and the range of quantitative detection was between 30ng/L to 3μg/L,with a good linearity,which will well potentially suit for sensitive analysis for MC-LR in drinking as well as surface water samples.; 国家高技术研究发展计划(863)项目(2002AA649160)
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/65267]
专题	清华大学
推荐引用方式 GB/T 7714	盛建武,何苗,施汉昌,等. 微囊藻毒素-LR多克隆抗体的制备[J],2010, 2010.
APA	盛建武.,何苗.,施汉昌.,钱易.,SHENG Jian-wu.,...&QIAN Yi.(2010).微囊藻毒素-LR多克隆抗体的制备..
MLA	盛建武,et al."微囊藻毒素-LR多克隆抗体的制备".(2010).

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