| 基于丝网印刷工艺的安培型酶免疫传感器的研究 | |
| 蔡强 ; 施汉昌 ; 何苗 ; 郭颖 ; 陈向强 ; Cai Qiang ; Shi Hanchang ; He Miao ; Guo Ying ; Chen Xiangqiang | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 安培法 免疫检测 丝网印刷电极 辣根过氧化物酶 Amperometric sensor,immunoassay,screen printed electrode,horseradish peroxidase O657.1 TP212.2 |
| 其他题名 | Amperometric Enzyme Immunosensor based on Screen Printing Technique |
| 中文摘要 | 研究了一种一次性安培型免疫传感器,检测辣根过氧化物酶(HRP)。用丝网印刷工艺制备三电极结构的传感器,工作电极是碳电极,银电极是伪参比电极。采用电荷传递系数、评估厚膜电极的一致性。通过戊二醛交联法,把抗HRP酶抗体固定在电极表面。加入底物H2O2和电子介体邻苯二胺,分别用安培法和比色法进行检测和比较分析。安培法测定HRP的测量范围10μg/L~10 mg/L,检出限10μg/L;比色法测定范围为2~40 mg/L,检出限2 mg/L。同时分析了湿度对传感器寿命的影响。; A disposale amperometric immunosensor which is fabricated by screen printing technique for the detection of horseradish peroxidase(HRP).Three-electrode structure was designed with carbon working(electrode) and silver pseudo-reference electrode.The reproducibility of those transducers was assessed by(electron) transfer coefficient based on cyclic voltammetry in ferrocene solution.Anti-HRP antibody was immobilized on the carbon electrode using glutaraldehyde.After binding with HRP,the response of immunosensor was determined by amperometric and colorimetric methods in substrate solution containing H_2O_2 and o-Phenylenediamine.The results show that the limit of detection for HRP is about 10 μg/L in amperometric method and 2 mg/L in colorimetric method.; 国家高技术研究发展计划(No.2002AA649160); 中国博士后基金(No.023208004)资助项目 |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/65243]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 蔡强,施汉昌,何苗,等. 基于丝网印刷工艺的安培型酶免疫传感器的研究[J],2010, 2010. |
| APA | 蔡强.,施汉昌.,何苗.,郭颖.,陈向强.,...&Chen Xiangqiang.(2010).基于丝网印刷工艺的安培型酶免疫传感器的研究.. |
| MLA | 蔡强,et al."基于丝网印刷工艺的安培型酶免疫传感器的研究".(2010). |
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