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	利用LIVE/DEAD Baclight染色测定活性污泥中的活菌水平
	梁鹏 ; 黄霞 ; LIANG Peng ; HUANG Xia
	2010-06-10 ; 2010-06-10
关键词	活性污泥 Baclight染色法 活菌 activated sludge,Baclight,live bacteria. X172
其他题名	THE LIVE/DEAD BACLIGHT STAINING METHOD FOR MONITORING THE LEVEL OF LIVE BACTERIA IN ACTIVATED SLUDGE
中文摘要	以LIVE/DEADBaclight染色法测定活性污泥中的活菌水平,采用荧光染色剂SYTO9和碘化丙啶(PI)对活性污泥进行染色,并利用荧光分光光度计测量绿色荧光(Fg,波长500—510nm)光强来表征活性污泥中活菌水平.该法直接对稀释后的污泥悬浊液进行测量,简化了测量步骤.当污泥浓度小于100mg·l-1时,每毫升样品需投加染色剂25μl;污泥浓度稀释到50mg·l-1,其惰性颗粒物对测量产生的影响在7%以下;超声波分散的最佳条件是:超声时间2min,功率40W.; The LIVE/DEAD Baclight staining method was used to measure the live bacteria ratio in the activated sludge. The sludge samples were stained by SYTO9 and PI(propidium iodide). The density of green fluorescence(Fg), which was measured by fluorescence spectroscopy, was used to indicate the level of live bacteria in the samples stained by SYTO9 and PI (propidium iodide). When the sludge concentration was lower than 100 mg·l-1, the dose of staining material was 25μl per milliliter sample. When the concentration of diluted activated sludge was lower than 50 mg·l-1, the affection of inert floc was lower than 7 %, which could be ignored. The sample dispersed by ultrasonic (the duration was 2 min and the power was 40 W) could improve the repeatability of the method.; 高等学校博士学科点专项科研基金(20020003041)资助项目
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/65134]
专题	清华大学
推荐引用方式 GB/T 7714	梁鹏,黄霞,LIANG Peng,等. 利用LIVE/DEAD Baclight染色测定活性污泥中的活菌水平[J],2010, 2010.
APA	梁鹏,黄霞,LIANG Peng,&HUANG Xia.(2010).利用LIVE/DEAD Baclight染色测定活性污泥中的活菌水平..
MLA	梁鹏,et al."利用LIVE/DEAD Baclight染色测定活性污泥中的活菌水平".(2010).

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