| 水体中微囊藻毒素-LR的间接竞争ELISA检测 | |
| 盛建武 ; 何苗 ; 余少青 ; 施汉昌 ; 钱易 ; SHENG Jian-wu ; HE Miao ; YU Shao-qing ; SHI Han-chang ; QIAN Yi | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 微囊藻毒素-LR 单克隆抗体 间接竞争ELISA 检测 microcystin-LR monoclonal antibody indirect competitive ELISA detection X132 |
| 其他题名 | Detection of Microcystin-LR in Waters Using Indirect Competitive ELISA |
| 中文摘要 | 在自制包被完全抗原浓度为5μg/mL、单克隆抗体工作稀释度为1∶3 000、酶标二抗鼠工作稀释度为1∶3 000、微囊藻毒素-LR浓度在0.001~30μg/L、显色底物为邻苯二胺,采用间接竞争酶联免疫吸附试验对水体中的微囊藻毒素-LR进行检测,结果表明,该方法与高效液相色谱的检测结果相关系数大于0.99,多次重复实验相对标准偏差小于10%,最低检测限能达到0.01μg/L,定量检测区间为0.01~3μg/L,该方法对[4-精氨酸]微囊藻毒素能特异性识别,对来自实际水样中的干扰有相当的耐受力.; Indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) was established to detect microcystin-LR in waters,with the concentration of the complete antigen was 5μg/mL,the dilution of the monoclonal antibody was 1∶(3?000), the dilution of the enzyme tracer(goat anti-rabbit IgG-peroxidase) was 1∶(3?000),the concentration range of microcystin-LR was between 0.001～30μg/L,and using o-phenylenediamine as substrate.The assay showed a high relativity of more than 99% with high performance liquid chromatography,a mean relative standard deviation less than 10%,a detection limitation under 0.01μg/L and quantitative detection range was 0.01～3μg/L,high specificity for [4-arginine] microcystin,and it could still perform well under the influence from the samples.; 国家高技术研究发展计划(863)项目(2002AA649160) |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/65045]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 盛建武,何苗,余少青,等. 水体中微囊藻毒素-LR的间接竞争ELISA检测[J],2010, 2010. |
| APA | 盛建武.,何苗.,余少青.,施汉昌.,钱易.,...&QIAN Yi.(2010).水体中微囊藻毒素-LR的间接竞争ELISA检测.. |
| MLA | 盛建武,et al."水体中微囊藻毒素-LR的间接竞争ELISA检测".(2010). |
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