| 质粒pT7降解三氯苯的功能研究 | |
| 宋蕾 ; 王慧 ; 施汉昌 ; SONG Lei ; WANG Hui ; SHI Han-chang | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 施氏假单胞菌 质粒 三氯苯 降解途径 Pseudomonas Stutzeri plasmid trichlorobenzene degradation pathwaty X172 |
| 其他题名 | The function study on trichlorobenzene degradation by plasmid pT7 |
| 中文摘要 | 从以1,2,4-三氯苯为唯一碳源的降解菌--施氏假单胞菌(Pseudomonas Stutzeri)T7中提取到一个降解质粒,为研究该质粒的降解功能,将其转化到E.coli JM109中,转化子获得了降解1,2,4-三氯苯的能力.采用色谱/质谱联用(GC/MS)技术检测到转化子E.coli JM109(含质粒pT7)降解1,2,4-三氯苯过程中的部分中间产物分别为2,3,5-三氯-2,4-己二烯二酸、2,5-二氯-2,4-二烯-1,4-内酯-己酸、2,5-二氯-4-氧代-2-烯己二酸;在转化子中分别检测了三氯苯双加氧酶、脱氢酶、邻苯二酚1,2-双加氧酶的酶活性.; A degradation plasmid was drawn in Pseudomonas Stutzeri T7 using 1,2,4-trichlorobenzene (1,2,4-TCB) as the degradation bacteria of only carbon source. For studying the function of this plasmid, it was transformed in E. coli JM109, thus obtaing the ability to degrade 1,2,4-TCB. In the process of E. coli JM109 (containing plasmid pT7) degrading 1,2,4-TCB, the partial intermediate products were inspected and surveyed as 2,3,5-trichloromuconate, 2,5-dichloro- carboxymethylenebut-2-en-4-olide, and 2,5-dichloro-4-oxohex-2-enedioate respectively, adopting gas chromatography-mass spectrometry (GC-MS) technique. The enzyme activities of trichlorobenzene dioxygenase, dihydrodiol dehydrogenase, and catechol 1,2-dioxygenase, the enzymes were inspected and surveyed respectively.; 国家“973”项目(2004CB418506); 清华大学基础研究基金资助(JC2003011) |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/65033]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 宋蕾,王慧,施汉昌,等. 质粒pT7降解三氯苯的功能研究[J],2010, 2010. |
| APA | 宋蕾,王慧,施汉昌,SONG Lei,WANG Hui,&SHI Han-chang.(2010).质粒pT7降解三氯苯的功能研究.. |
| MLA | 宋蕾,et al."质粒pT7降解三氯苯的功能研究".(2010). |
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