QQ客服 官方微博 反馈留言

	3-羟丙醛对Klebsiella pneumoniae发酵产1,3-丙二醇的影响及其调控
	林日辉 ; 刘宏娟 ; 张君 ; 郝健 ; 程可可 ; 刘德华 ; LIN Ri-hui ; LIU Hong-juan ; ZHANG Jun ; HAO Jian ; CHENG Ke-ke ; LIU De-hua
	2010-06-10 ; 2010-06-10
关键词	Klebsiellapneumoniae 1,3-丙二醇 3-羟丙醛 调控 1,3-propanediol Klebsiella pneumoniae 3-hydroxypropionaldehyde regulation TQ223.162
其他题名	Effect of 3-hydroxypropionaldehyde on 1,3-propanediol fermentation by Klebsiella pneumoniae and regulation for it
中文摘要	中间产物3-羟丙醛在发酵液中的积累对Klebsiella pneumoniae细胞生长及1,3-丙二醇的合成有显著的抑制作用,而调节发酵的起始甘油浓度及控制发酵pH值可调控发酵液中3-羟丙醛的积累。当起始甘油质量浓度分别为20、30、50、70 g/L的批式发酵中,发酵液中3-羟丙醛的积累的高峰分别为4.31、6.871、1.48及13.49 mmol/L,当起始甘油质量浓度大于50 g/L时,3-羟丙醛在到达积累高峰后不能被菌体有效转化,在发酵后期维持较高浓度,抑制了细胞生长及1,3-丙二醇的合成,发酵不能继续进行。控制发酵pH值为7.75~8.0可促进发酵液堆积的3-羟丙醛被迅速转化。在流加发酵中起始甘油质量浓度采用30 g/L,发酵pH值控制为7.75条件下,发酵32 h,1,3-丙二醇质量浓度可达37.16 g/L,1,3-丙二醇的生产强度和质量得率分别达到1.16 g/(L.h)和52.66%。; 3-Hydroxypropionaldehyde,an intermediate product during the 1,3-propanediol fermentation by Klebsiella pneumoniae,can inhibite cell growth and 1,3-propanediol production,but it can be controlled by regulating the initial glycerol concentration and pH value.In the batch culture with the initial glycerol concentration of 20,30,50 and 70 g/L,(3-hydroxypropionaldehyde) concentration can reach the peak of 4.31,6.87,11.48 and 13.49 mmol/L respectively.The 3-hydroxypropionaldehyde accumulated can't be converted effectively when the initial glycerol concentration is higher than (50 g/L),which led to the fermentation ceasing.A proper pH controlling strategy (pH=7.75-8.0) can enhance the conversion of 3-hydroxypropionaldehyde in the broth and decrease the toxicity of 3-hydroxypropionaldehyde to the cell.During a fed-batch culture with the initial glycerol concentration of 30 g/L at a pH value of 7.75,1,3-propanediol concentration reached 37.16 (g/L) in (32 h) of fermentation.Furthermore,the productivity and yield can be 1.16 g/(L穐) and 52.66% (g/g) respectively.; “十五”国家科技攻关计划资助项目(2001BA708B01-04)
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/64511]
专题	清华大学
推荐引用方式 GB/T 7714	林日辉,刘宏娟,张君,等. 3-羟丙醛对Klebsiella pneumoniae发酵产1,3-丙二醇的影响及其调控[J],2010, 2010.
APA	林日辉.,刘宏娟.,张君.,郝健.,程可可.,...&LIU De-hua.(2010).3-羟丙醛对Klebsiella pneumoniae发酵产1,3-丙二醇的影响及其调控..
MLA	林日辉,et al."3-羟丙醛对Klebsiella pneumoniae发酵产1,3-丙二醇的影响及其调控".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。