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	D-对羟基苯甘氨酸生产过程的高效毛细管电泳法分析
	贾宁 ; 李强 ; 王亚辉 ; JIA Ning ; LI Qiang ; WANG Ya-Hui
	2010-06-10 ; 2010-06-10
关键词	D-对羟基苯甘氨酸 DL-对羟基苯海因 N-氨甲酰基-D-对羟基苯甘氨酸 高效毛细管电泳 测定 D-p-hydroxyphenylglycine DL-hydroxyphenylhydantoin N-carbamoyl-D-p-hydroxyphenylglycine HPCE determination TQ245.6
其他题名	Determination of D-p-hydroxyphenylglycine by HPCE
中文摘要	建立了高效毛细管电泳法测定酶法反应体系中D-对羟基苯甘氨酸、DL-对羟基苯海因、N-氨甲酰基-D-对羟基苯甘氨酸的含量。采用未涂层石英毛细管柱,运行缓冲液为20mmol/L硼砂缓冲液(pH9.0),检测波长230nm。分离电压30kV,进样压力0.5psi,进样时间5s,采用苯酚为内标。三成分均在0.01%～0.1%范围内线性关系良好。; A high performance capillary electrophoresis was established for the determination of D-p- hydroxyphenylglycine, DL-hydroxyphenylhydantoin and N-carbamoyl-D-p-hydroxyphenylglycine in the course of enzymatic production. An uncoated fused silica capillary column was used with the running buffer solution of 20mmol/L borate buffer solution(pH 9.0), at the detection wavelength of 230nm. The separation voltage was 30kV, with inject pressure of 0.5psi and inject time of 5s. Phenol was used as internal standard. The linear ranges of the three compounds were 0.01% - 0.1%.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/64351]
专题	清华大学
推荐引用方式 GB/T 7714	贾宁,李强,王亚辉,等. D-对羟基苯甘氨酸生产过程的高效毛细管电泳法分析[J],2010, 2010.
APA	贾宁,李强,王亚辉,JIA Ning,LI Qiang,&WANG Ya-Hui.(2010).D-对羟基苯甘氨酸生产过程的高效毛细管电泳法分析..
MLA	贾宁,et al."D-对羟基苯甘氨酸生产过程的高效毛细管电泳法分析".(2010).

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