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	细菌Enterobacter dissolvens的直流电解刺激过程
	佘鹏 ; 宋波 ; 邢新会 ; 刘铮 ; SHE Peng ; SONG Bo ; XING Xin-hui ; LIU Zheng
	2010-06-10 ; 2010-06-10
关键词	直流电 电解刺激 Enterobacterdissolvens 脱氢酶 葡萄糖 氧化还原电位 direct current electrolytic stimulation Enterobacter dissolvens dehydrogenase activity glucose redox potential Q937
其他题名	Electrolytic Stimulation of Enterobacter dissolvens Cultivated in Glucose Medium
中文摘要	以葡萄糖为唯一碳源,研究了细菌Enterobacterdissolvens在直流电条件下的生长和代谢过程.实验结果表明,当使用盐桥屏蔽电极反应时,细胞生长曲线和葡萄糖代谢速率基本不受电流强度的影响,而当采用铂丝电极时,反应体系中发生水解反应,采用10mA电流通电12h后,菌液中细胞的脱氢酶比活力和葡萄糖降解率分别为对照的1.98倍和1.48倍,细胞生长亦有所加快,但在其活力下降时出现大量死亡.扫描电镜照片显示细胞结构受到明显破坏,并发生细胞膜穿孔现象,这表明可能受到了过氧化氢等阳极水解中间产物的刺激作用.; Growth and metabolic behavior of E. dissolvens under the exposure to weak direct current (DC) was investigated by using glucose as the sole carbon source. When salt bridge electrodes were applied to shield the electrode reactions, the implementation of DC led to neglectable increases in cell growth and glucose uptake. According to the cyclic voltammogram of cell broth, water electrolysis was determined to be the main electrode reaction when DC was implemented via platinum electrodes. After 12 h electrolytic stimulation, the specific dehydrogenase activity and glucose consumption was 1.98 and 1.48 fold as much as that determined in the absence of electric field, respectively. These might be due to the generation of the hydrogen at cathode, which decreased the redox potential of culture solution and maintained a reductive environment even under aerobic condition. The enhanced cell growth could be seen as a result of increased glucose uptake under the application of DC. Accelerated cell death was also observed during the later stationary phase. Scanning electron microscopic observation showed that the cells after the growth phase were damaged by the DC application.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/64277]
专题	清华大学
推荐引用方式 GB/T 7714	佘鹏,宋波,邢新会,等. 细菌Enterobacter dissolvens的直流电解刺激过程[J],2010, 2010.
APA	佘鹏.,宋波.,邢新会.,刘铮.,SHE Peng.,...&LIU Zheng.(2010).细菌Enterobacter dissolvens的直流电解刺激过程..
MLA	佘鹏,et al."细菌Enterobacter dissolvens的直流电解刺激过程".(2010).

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