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	高效液相色谱分析法测定色氨酸对映体
	陈扬 ; 于燕梅 ; 骆广生 ; CHEN Yang ; YU Yan-mei ; LUO Guang-sheng
	2010-06-10 ; 2010-06-10
关键词	高效液相色谱 色氨酸对映体 手性冠醚 high performance liquid chromatography tryptophan enantiomers chiral crown ether O657.72
其他题名	High Performance Liquid Chromatographic Analysis of Tryptophan Enantiomers
中文摘要	采用高效液相色谱法,以手性冠醚Crownpak CR(+)为固定相、高氯酸溶液/甲醇混合溶液为流动相,成功地实现了色氨酸(Trp)对映体的色谱分离.考察了流动相中甲醇含量、流量和柱温等因素对分离效果的影响.研究表明,适当提高流动相中的甲醇含量、降低流动相流量、降低柱温可以有效地提高分离度.另一方面,提高流动相中的甲醇含量、提高流动相流量、升高柱温可以显著地缩短样品的保留时间.确定了一种最佳分析分离条件,即流动相为甲醇∶高氯酸溶液=15∶85(V/V),流动相流量0.8 mL/min,柱温20℃,波长220 nm,在此条件下进行色谱分离,样品的保留时间小于12 min,分离度达到2.06.该方法具有快速、高效、准确和精密度高等优点.; A high performance liquid chromatographic(HPLC) separation of tryptophan(Trp) enantiomers was successfully realized by using chiral crown ether [Crownpak CR(+)] as the immobile phase and aqueous perchloric acid solution mixed with methanol as the mobile phase.The influences of methanol content in the mobile phase,the flow flux of the mobile phase and the column temperature on the separation performance were investigated.The resolution ability can be improved respectively by increasing methanol content in the mobile phase in a certain extent,by decreasing the flux of the mobile phase or the column temperature.The retention time of samples can be significantly shorten by increasing methanol content in the mobile phase,by increasing the flux of the mobile phase or the column temperature.The optimal conditions for analysis and separation were established with methanol: perchloric acid=15∶85(V/V) as mobile phase at the flow flux of 0.8 mL/min,the UV wavelength of 220 nm and the column temperature of 20 ℃.Under the above conditions,the retention time of samples was less than 12 min and the separation factor can reach 2.06.The proposed method had advantages of short analysis time,high resolution,high accuracy as well as high precision.; 国家自然科学基金(20525622,20676066)
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/64104]
专题	清华大学
推荐引用方式 GB/T 7714	陈扬,于燕梅,骆广生,等. 高效液相色谱分析法测定色氨酸对映体[J],2010, 2010.
APA	陈扬,于燕梅,骆广生,CHEN Yang,YU Yan-mei,&LUO Guang-sheng.(2010).高效液相色谱分析法测定色氨酸对映体..
MLA	陈扬,et al."高效液相色谱分析法测定色氨酸对映体".(2010).

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