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	空间环境对恶性黑色素瘤B16细胞间通讯功能的影响
	柴大敏 ; 向青 ; 徐梅 ; 徐波 ; 李红艳 ; 耿传营 ; 陈志华 ; 陶仪声 ; 唐劲天 ; CHAI Damin ; XIANG Qing ; XU Mei ; XU Bo ; LI Hongyan ; GENG Chuanying ; CHEN Zhihua ; TAO Yisheng ; TANG Jintian
	2010-06-10 ; 2010-06-10
关键词	空间环境 B16细胞 诱变 CD31 细胞间隙通讯功能 outer space environment B16 cell mutagenesis CD31 gap junction intercellular communication R85
其他题名	Gap Junction Intercellular Communications of Melanoma B16 Cell Clones Mutagenized by Outer Space Environment
中文摘要	观察空间诱变后恶性黑色素瘤B16细胞株的细胞间通讯功能及细胞间黏附能力的变化,并探讨可能的机理。应用细胞低温长期生存系统,将B16细胞株搭载于“第20颗返回式卫星”,返地后单克隆化,从得到的110株单克隆空间诱变B16细胞株中随机选取6株,编号为39#～44#,常规培养6代后和对照细胞株同时做MTT和FCM,检测细胞增殖和周期分布;用划痕标记染料示踪技术检测细胞间通讯功能;将有变异的细胞接种C57BL/6J小鼠,免疫组化观测CD31蛋白的表达量。初步筛选出两株(39#、44#)变化比较明显的细胞株,44#细胞增殖能力降低、G1期分布增加、细胞间通讯功能增强、CD31蛋白表达增强;39#细胞增殖能力升高、G1期分布减少、细胞间通讯功能减弱、CD31蛋白表达减少。空间环境的作用使体外培养肿瘤细胞产生多向变异,有可能筛选出有意义的细胞株做进一步的研究。; To study the gap junction intercellular communication and adhension of the melanoma B16 cell clones mutagenized by space environment,and the possible mechanism.B16 cells were put on board of the Chinese 20th recoverable satellite,and were harvested after 18 days' spaceflight and then monocloned.Six cell clones numbered as 39#～44# were randomly selected from the total 110 clones obtained,and the control clones were routinely cultured.After six generations,the cultured cells were experimented with MTT assay and FCM methods for cell growth characteristics;with scrape loading dye transfer method for gap junction interceluler communication;with immunohistochemistry method for the CD31 contents.Two clones were selected as remarkably different from the control clone in growth characteristics,gap junction intercellular communication,intercellular adhension.Cultured melanoma B16 cells in vitro were found to be mutated by the outer space environment.One may screen out mutagensis B16 cell clones to carry out studies useful for human beings.; 国家自然科学基金项目(No.30371324); 国家科技部973计划前期项目[2003(CA04200)]
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/63749]
专题	清华大学
推荐引用方式 GB/T 7714	柴大敏,向青,徐梅,等. 空间环境对恶性黑色素瘤B16细胞间通讯功能的影响[J],2010, 2010.
APA	柴大敏.,向青.,徐梅.,徐波.,李红艳.,...&TANG Jintian.(2010).空间环境对恶性黑色素瘤B16细胞间通讯功能的影响..
MLA	柴大敏,et al."空间环境对恶性黑色素瘤B16细胞间通讯功能的影响".(2010).

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