| 细胞太空无源搭载装置中的微载体技术初步研究 | |
| 房青 ; 徐波 ; 徐梅 ; 向青 ; 李红艳 ; 陈志华 ; 赵天德 ; 唐劲天 ; FANG Qing ; XU Bo ; XU Mei ; XIANG Qing ; LI Hongyan ; CHEN Zhihua ; ZHAO Tiande ; TANG Jintian | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 微载体 空间 细胞 核酸 micro-carrier space cell nucleic acids Q2-33 |
| 其他题名 | A Preliminary Study on Micro-carrier Technique in the Space Cell Culture System |
| 中文摘要 | 探讨基于微载体技术的改良细胞单克隆化方法,及直接研究微载体上细胞形态、细胞增殖、核酸提取的方法。利用快速染色法直接观察微载体上的细胞形态,并用MTT法直接分析细胞增殖,用Trizol试剂和吸附柱直接从微载体提取细胞核酸。对微载体上的细胞可直接进行染色,观察正常细胞和凋亡细胞的形态。MTT分析显示细胞数和OD值呈直线关系。从微载体上直接提取的RNA完整性好,可用于RT-PCR分析,提取的DNA含量较高。改良细胞单克隆化方法操作简便,细胞活性好;不消化细胞,可以直接研究微载体上的细胞形态、细胞增殖,提取核酸。; In this paper,a refined cell monocolonization protocol is explored,together with methods of obtaining the cell shape and proliferation,and the method of isolation of nucleic acids from cells on micro-carriers.Without dissociating cells with trysin from micro-carrier,morphological characteristics of normal and apoptotic cells are analyzed by staining and microscopic observation,while the cell proliferation is analyzed by MTT methods.The RNA and DNA are directly isolated with Trizol reagent and QIAamp DNA kit from cells adhered on micro-carriers.Cells can be morphologically observed and photographed when attached to micro-carrier.MTT analysis shows that a linear relation exists between cell amount and OD value.RNA isolated from cells on micro-carriers is intact and can be used for RT-PCR.DNA quantity of directly isolated group is larger than that of trysin group.Cell monocolonization protocol modified by us is simpler and cheaper with acceptable viability.The shape and proliferation of cells attached on micro-carriers can be directly observed,and nucleic acids can be isolated.; 国家自然科学基金项目(30371324); 科学技术部973计划前期项目[2003(CA04200)] |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/63152]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 房青,徐波,徐梅,等. 细胞太空无源搭载装置中的微载体技术初步研究[J],2010, 2010. |
| APA | 房青.,徐波.,徐梅.,向青.,李红艳.,...&TANG Jintian.(2010).细胞太空无源搭载装置中的微载体技术初步研究.. |
| MLA | 房青,et al."细胞太空无源搭载装置中的微载体技术初步研究".(2010). |
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