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	经羟基磷灰石处理的纯钛表面有利于角膜成纤维细胞的粘附和增殖(英文)
	马骁 ; 李朝辉 ; 黄一飞 ; 陆玉杰 ; 王乐云 ; 黄靖香 ; Xiao Ma ; Zhao-Hui Li ; Yi-Fei Huang ; Yu-Jie Lu ; Le-Yun Wang ; Jing-Xiang Huang
	2010-06-10 ; 2010-06-10
关键词	羟基磷灰石 表面改性 人工角膜 角膜基质成纤维细胞 粘附 增殖 hydroxyapatite, titanium,keratoprosthesis, corneal fibroblasts. R779.64
其他题名	Hydroxyapatite modified titanium promotes superior adhension and proliferation of corneal fibroblast in comparison with pure titanium
中文摘要	目的:研究与纯钛相比,经羟基磷灰石表面改性处理的纯钛是否能增加兔角膜基质成纤维细胞在其表面的粘附和增殖。方法:利用生物活性的羟基磷灰石对纯钛进行表面改性。将第4代兔角膜基质成纤维细胞接种于羟基磷灰石表面改性的钛、纯钛及玻璃表面,24,48,72h后,使用丫啶橙染色的方法观察材料表面细胞的粘附和增殖情况,电镜观察材料表面的细胞形态。结果:各个时间点,羟基磷灰石表面改性的钛表面的细胞数都多于其他材料(P<0.05)。24h,羟基磷灰石表面改性的钛表面和玻璃表面的细胞扩展面积要大于纯钛表面。72h电镜观察发现羟基磷灰石表面改性的钛表面的细胞扩展面积最大,细胞张力丝最长。结论:与纯钛相比,经羟基磷灰石表面改性处理的纯钛可增加兔角膜基质成纤维细胞在其表面粘附和增殖; � AIM: To determine whether hydroxyapatite modified titanium promotes superior adhension and proliferation of rabbit corneal fibroblast in comparison with pure titanium. � METHODS: We used bioactive hydroxyapatite to modify titanium surfaces. Fourth passage fibroblasts of rabbit cornea were seeded on hydroxyapatite modified titanium surfaces, pure titanium and glass surfaces. Cell adhension, proliferation and morphology were detected at 24 hours, 48 hours, and 72hours using a acridine orange stain. Further studies of cell morphology were performed using scanning electron microscopy. � RESULT: Cell counts were significantly greater on hydroxyapatite modified titanium surfaces at each time point(P<0.05). At 24 hours, cell spreading was greater on hydroxyapatite-coated titanium and glass than on the pure titanium. At 72 hours, compared with pure titanium and glass surfaces, the cells on hydroxyapatite modified titanium surfaces had greater spreading area and longer stress fibers. � CONCLUSIONS: Hydroxyapatite modified titanium promotes superior adhension and proliferation of rabbit corneal fibroblast in comparison with pure titanium. �; 中国人民解放军十五(No.01MA109); 十一五(No.06MA281)课题资助项目~~
语种	英语 ; 英语
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/61555]
专题	清华大学
推荐引用方式 GB/T 7714	马骁,李朝辉,黄一飞,等. 经羟基磷灰石处理的纯钛表面有利于角膜成纤维细胞的粘附和增殖(英文)[J],2010, 2010.
APA	马骁.,李朝辉.,黄一飞.,陆玉杰.,王乐云.,...&Jing-Xiang Huang.(2010).经羟基磷灰石处理的纯钛表面有利于角膜成纤维细胞的粘附和增殖(英文)..
MLA	马骁,et al."经羟基磷灰石处理的纯钛表面有利于角膜成纤维细胞的粘附和增殖(英文)".(2010).

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