QQ客服 官方微博 反馈留言

	局部抗菌药物载药系统克林霉素磷酸钙水泥的实验研究
	储诚兵 ; 黎檀实 ; 郝立波 ; 王慧 ; 曹敬荣 ; 王继芳 ; CHU Cheng-bing ; LI Tan-shi ; HAO Li-bo ; WANG Hui ; CAO Jing-rong ; WANG Ji-fang
	2010-06-10 ; 2010-06-10
关键词	磷酸钙水泥 克林霉素 抗菌药物 载药系统 Calcium phosphate cement Clindamycin Antibiotic Delivery system R94
其他题名	Clindamycin-loaded Calcium Phosphate Cement as Local Antibiotic Delivery System
中文摘要	目的探讨磷酸钙水泥(CPC)复合克林霉素制成局部抗菌药物载药系统的可行性。方法观察CPC复合2%、5%克林霉素后,对初凝时间(tI)及终凝时间(tF)的影响;高效液相色谱法(HPLC)测定2%、5%复合克林霉素磷酸钙水泥(CLCPC)试样浸于PBS液中每日释放克林霉素的浓度及持续时间;采用平皿扩散法观察2%、5%CLCPC及2%复合克林霉素Palacos骨水泥(CLPMMP)体外抑菌能力;X射线晶体衍射(XRD)分析及扫描电镜(SEM)观察克林霉素对CPC固化产物及结构有无影响。结果克林霉素缩短了2%、5%CLCPC的固化时间;2%5、%CLCPC释放克林霉素在最初6 h呈暴发性,到第4天克林霉素释放速度减慢,至42 d仍能释放克林霉素;2%CLPMMP比2%CLCPC的每日抑菌环小,2%CLCPC又比5%CLCPC的抑菌环小;至抑菌试验42 d,CLCPC及CLPMMP仍均有抑菌作用;从抑菌试验30 d起,放置2%CLPMMP试样处可见大量细菌菌落,而2%、5%CLCPC无此现象;XRD分析及SEM观察克林霉素对CPC固化产物、晶体大小及结构无影响。结论克林霉素可复合CPC制成局部抗菌药物载药系统。; OBJECTIVE To determine the feasibility of clindamycin-loaded calcium phosphate cement(CLCPC) as a local antibiotic delivery system.METHODS The initial setting time(tI) and the final setting time(tF) were measured for 0%,2% and 5% CLCPC according to ASTM C266-89 method.Clindamycin concentrations eluting from the samples of 2% and 5% CLCPC in PBS were analyzed by HPLC at different times.The bacteriostasis tests were done by plate diffusion method for 2% and 5% CLCPC and 2% Palacos R-40 bone cement(PMMP) samples,and the diameters of the bacteriostasis ring and bacteriostasis duration were observed.The setting product and crystal size of 0%,2% and 5% CLCPC were analyzed and observed by X-ray diffraction(XRD) and scanning electron microscopy(SEM).RESULTS The setting time could be shortened by adding clindamycin(tI,tF) of 2% and 5% CLCPC.Clindamycin was with burst-release from CLCPC within the intial 6-hour period and the release rate slowed down on 4th day.Clindamycin could still release until to the 42th day.The ring of 2% Palacos R-40 bone cement(PMMP) bacteriostasis was smaller than that of 2% CLCPC,and the ring of 2% CLCPC bacteriostasis was smaller than that of 5%CLCPC.The bacteriostasis still existed to the 42th day of test for 2% and 5%CLCPC and 2% Clindamycin-loaded Palacos R-40 bone cement(CLPMMP).From the 30th day,many bacterial colonies were seen in the culture media laying 2%CLPMMP sample.On the contrary,bacterial colony was not found in the media putting 2% and 5%CLCPC.XRD and SEM showed that clindamycin did′t have an influence on setting product,crystal size and structure of CPC.CONCLUSIONS Clindamycin-loaded calcium phosphate cement can be used as a local antibiotic delivery system.; 国家自然科学基金项目(39670731)
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/61423]
专题	清华大学
推荐引用方式 GB/T 7714	储诚兵,黎檀实,郝立波,等. 局部抗菌药物载药系统克林霉素磷酸钙水泥的实验研究[J],2010, 2010.
APA	储诚兵.,黎檀实.,郝立波.,王慧.,曹敬荣.,...&WANG Ji-fang.(2010).局部抗菌药物载药系统克林霉素磷酸钙水泥的实验研究..
MLA	储诚兵,et al."局部抗菌药物载药系统克林霉素磷酸钙水泥的实验研究".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。