| 纳米羟基磷灰石/胶原对富血小板血浆促进骨髓基质干细胞成骨向分化的影响 | |
| 王程越 ; 姚玉胜 ; 艾红军 ; 崔福斋 ; WANG Cheng-yue ; YAO Yu-sheng ; AI Hong-jun | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 纳米羟基磷灰石/胶原 富血小板血浆 骨髓基质干细胞 Nano-hydroxyapatite/collagen Platelet-rich plasma Bone marrow stromal cells R329 |
| 其他题名 | Effects of Nano-hydroxyapatite/collagen on Differentiation of Bone Marrow Stromal Cells into Osteoblasts Induced by Platelet-rich Plasma in Vitro |
| 中文摘要 | 目的:探讨纳米羟基磷灰石/胶原(nHAC)作为支架材料对兔自体富血小板血浆(PRP)体外诱导兔骨髓基质干细胞(rBMSCs)向成骨细胞分化能力的影响。方法:兔自体PRP分别作用于与nHAC联合培养的rBMSCs及常规培养的rBMSCs,利用扫描电镜观察rBMSCs在nHAC上的生长情况;通过碱性磷酸酶(ALP)活性测定,骨钙素(OCN)含量测定,骨桥蛋白(opn)mRNA相对表达量的分析,比较两种培养条件下兔自体PRP诱导rBMSCs向成骨细胞分化能力上的差异。结果:联合培养的rBMSCs在nHAC上生长良好,其ALP活性、OCN含量均较常规培养明显增加,且opnmRNA相对表达量是常规培养组的4.78倍。结论:nHAC作为支架材料可明显提高兔自体PRP诱导rBMSCs向成骨细胞分化的能力。; Objective:To explore nano-hydroxyapatite/collagen(nHAC) and platelet-rich plasma(PRP) on inducing rabbit bone marrow stromal cells(rBMSCs) into osteoblasts.Methods:rBMSCs co-cultured with or without nHAC were induced by PRP.The growth state of rBMSCs co-cultured with nHAC was observed by scaning electron microscope.To compare with the ability of PRP inducing rBMSCs co-cultured with or without nHAC into osteoblasts,alkaline phosphatase(ALP) activity,osteocalcin(OCN) content and the mRNA level of osteopontin(OPN) were measured.Results:The osteoblasts differentiated from rBMSCs grew well on nHAC.Compared with the cells cultured conventionally,ALP activity and OCN content of cells co-cultured with nHAC increased greatly.The relative expression level of OPN mRNA under co-culture condition was 4.78 times greater than that of under conventional culture condition.Conclusion:nHAC as scaffold material could improve the ability of PRP to induce the rBMSCs into osteoblasts.; 辽宁省教育厅资助项目(编号:05L481) |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/61281]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 王程越,姚玉胜,艾红军,等. 纳米羟基磷灰石/胶原对富血小板血浆促进骨髓基质干细胞成骨向分化的影响[J],2010, 2010. |
| APA | 王程越.,姚玉胜.,艾红军.,崔福斋.,WANG Cheng-yue.,...&AI Hong-jun.(2010).纳米羟基磷灰石/胶原对富血小板血浆促进骨髓基质干细胞成骨向分化的影响.. |
| MLA | 王程越,et al."纳米羟基磷灰石/胶原对富血小板血浆促进骨髓基质干细胞成骨向分化的影响".(2010). |
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