| 结合两种LC/MS方法用于复方丹参滴丸中多成分的鉴定 | |
| 章弘扬 ; 胡坪 ; 梁琼麟 ; 罗国安 ; 王义明 ; ZHANG Hong-yang ; HU Ping ; LIANG Qiong-lin ; LUO Guo-an ; WANG Yi-ming | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 复方丹参滴丸 飞行时间质谱 离子阱多级质谱 多成分鉴定 Danshen Dropping Pills time-of-flight mass spectrometer ion trap tandem mass spectrometer multi-component identification R286 |
| 其他题名 | Identification of multi-component in Danshen Dropping Pills by using a combinative LC/MS approach |
| 中文摘要 | 目的:建立一种结合两种LC/MS的方法,对复方丹参滴丸(丹参,三七,冰片,等)中的多成分进行鉴定。方法:色谱分离采用Phenomenex Luna C18反相柱(250 mm×4.6 mm,5μm),流动相组成分别为0.2%甲酸水溶液和乙腈,梯度洗脱,流速:1.0 mL/min;质谱定性采用飞行时间质谱(TOF-MS)结合离子阱多级质谱(Trap-MSn),负离子模式扫描。结果:在优化的液质联用条件下,飞行时间质谱筛查出复方中14种成分的分子式,采用离子阱多级质谱共鉴定出13种成分,并对其药材来源进行了归属。结论:本实验建立了一种结合两种LC/MS的分析方法,为中药复方多成分的分析提供了一种有效、可靠的新模式,也同样适用于其它复杂体系的分析。; AIM: To develop a combinative LC/MS for the identification of multi-component in Danshen Dro-(pping) Pills(Radix et Rhizoma Salviae miltiorrhizale,Radix et Rhizoma Notoginseng,Borneolum Syntheticum,etc.). METHODS: Separation was performed on a Phenomenex Luna C_(18) reverse-phase column(250 mm×4.6 mm,5 μm).The mobile phase consisted of water containing 0.2% formic acid and acetonitrile was used as gradient elute.The flow rate was 1 mL/min.A combination of time-of-flight mass spectrometer(TOF-MS) and ion trap tandem mass spectrometer(Trap-MS~n) was applied for qualitative analysis under negative ion mode. RESULTS: Under optimized LC/MS condition,the molecular formulae of 14 components were screened by TOF-MS and 13 of them were then identified by Trap-MS~n.All of the components were surveyed and classified according to their medicinal materials derivation. CONCLUSION: The established combinative LC/MS method can thus be considered as an effective and credible pattern for the analysis of Chinese multi-herb remedy,as well as other complex systems.; 国家十一五科技支撑计划(2006BAI08B04-01) |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/60978]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 章弘扬,胡坪,梁琼麟,等. 结合两种LC/MS方法用于复方丹参滴丸中多成分的鉴定[J],2010, 2010. |
| APA | 章弘扬.,胡坪.,梁琼麟.,罗国安.,王义明.,...&WANG Yi-ming.(2010).结合两种LC/MS方法用于复方丹参滴丸中多成分的鉴定.. |
| MLA | 章弘扬,et al."结合两种LC/MS方法用于复方丹参滴丸中多成分的鉴定".(2010). |
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