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	反相高效液相色谱法同时测定川芎中的四种内酯类化合物
	曹建敏 ; 王宗花 ; 丁明玉 ; 杨学东 ; 寇怀江 ; CAO Jianmin ; WANG Zonghua ; DING Mingyu ; YANG Xuedong ; KOU Huaijiang
	2010-06-10 ; 2010-06-10
关键词	反相高效液相色谱法 洋川芎内酯-H 洋川芎内酯-I 瑟丹酸内酯 蒿本内酯 川芎 中草药 reversed-phase high performance liquid chromatography senkyunolide-H senkyunolide-I sedanenolide Z-ligustilide Chuanxiong Chinese herbal medicine O657.72
其他题名	Determination of Lactone Components in Chuanxiong by Reversed-Phase High Performance Liquid Chromatography
中文摘要	用反相高效液相色谱法同时测定了川芎中的4种主要内酯类成分(洋川芎内酯-H、洋川芎内酯-I、瑟丹酸内酯和蒿本内酯)。采用XDB-C8柱,以甲醇-1%乙酸水溶液为流动相进行梯度洗脱,甲醇-1%乙酸水溶液的体积比在15m in内从55∶45线性变化至100∶0,流速0.8m L/m in,紫外检测波长280nm。4种内酯类化合物的检测限为0.011~0.027μg,回收率为96%~108%。该方法操作简单,结果准确,重现性好,可用于川芎药材及饮片中4种内酯类化合物的同时测定,具有很高的实用价值。; A reversed-phase high performance liquid chromatographic method was developed for the simultaneous determination of four main lactone components including senkyunolide-H, senkyunolide-I, sedanenolide and Z-ligustilide in Chuanxiong, a Chinese herbal medicine. The samples were pretreated (using) ultrasonic extraction, and 80% ethanol was used as extractant in the experiment. The chromatographic analysis was carried out using an XDB-C_8 column and the mobile phase consisted of methanol-1%(volume fraction) acetic acid water (5545→1000, (v/v,) in 15 min). The flow rate was 0.8 mL/min. A diode array detector was used to detect the compositions and 280 nm was chosen as the detection wavelength. The whole process could be performed within 15 min. The operating curves were found to be linear over the ranges of 0.4-2.2 mg/L (r=(0.9992)), 0.4-2.2 mg/L (r=(0.9979)), 2.6-7.8 mg/L (r=(0.9992)) and 4.8-14.3 mg/L (r=(0.9998)) and the detection limits (S/N=3) were 0.024, 0.027, 0.018 and 0.011 μg for senkyunolide-H, senkyunolide-I, sedanenolide and Z-ligustilide, respectively. The recoveries of the four lactone components were found in the range of 96%-108%. The rapid and accurate method has been successfully applied to the simultaneous determination of the four lactone components in Chuanxiong.; 北京市科委重点项目(No.H037330010120)
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/60947]
专题	清华大学
推荐引用方式 GB/T 7714	曹建敏,王宗花,丁明玉,等. 反相高效液相色谱法同时测定川芎中的四种内酯类化合物[J],2010, 2010.
APA	曹建敏.,王宗花.,丁明玉.,杨学东.,寇怀江.,...&KOU Huaijiang.(2010).反相高效液相色谱法同时测定川芎中的四种内酯类化合物..
MLA	曹建敏,et al."反相高效液相色谱法同时测定川芎中的四种内酯类化合物".(2010).

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