| 偏端霉素衍生物与DNA相互作用的圆二色谱测定 | |
| 叶勇 ; 杨俊亮 ; 刘艳红 ; 赵玉芬 ; YE Yong ; YANG Junliang ; LIU Yanhong ; ZHAO Yufen | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 圆二色谱 偏端霉素衍生物 核酸 circular dichroism distamycin derivatives DNA R96 |
| 其他题名 | Investigation on interaction between distamycin derivatives and DNA by circular dichroism spectroscopy |
| 中文摘要 | 目的:检测生理pH值下偏端霉素衍生物与DNA间的相互作用。方法:单独的配体和DNA在300～380nm区域都没有信号,向DNA液中滴加偏端霉素衍生物,在圆二色谱(CD谱)上300～370nm区域会有信号出现。利用这种方法观察核酸构象的变化。结果:在偏端霉素衍生物与DNA的混合液的CD谱上观察到在300～370nm区域有特征峰。结论:偏端霉素衍生物对DNA的碱基有识别功能。; Aim:To detect the biological activity of new synthetic distamycin derivatives,the present work gives the results of studies on the interaction between distamycin derivatives and DNA under physiological pH.Methods:Neither the free DNA nor the free ligands exhibited circular dichroism(CD)signals in the 300~380 nm wavelength region.With incremental titration of distamycin derivatives,in the CD spectra of mixed solutions a specific absorption peak will appeare in the 300~380 nm wavelength region.By this method to study the structure change of DNA.Results:In the CD spectra of the mixing solution of DNA with distamycin derivatives a specific peak appeared in the region of 300~370 nm wavelength.Conclusion:The distamycin derivatives may have ability to recognize the base of DNA.; 国家自然科学基金资助项目20602032,20732004,20972143 |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/60782]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 叶勇,杨俊亮,刘艳红,等. 偏端霉素衍生物与DNA相互作用的圆二色谱测定[J],2010, 2010. |
| APA | 叶勇.,杨俊亮.,刘艳红.,赵玉芬.,YE Yong.,...&ZHAO Yufen.(2010).偏端霉素衍生物与DNA相互作用的圆二色谱测定.. |
| MLA | 叶勇,et al."偏端霉素衍生物与DNA相互作用的圆二色谱测定".(2010). |
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