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	人热休克蛋白70基因的克隆、表达及鉴定
	胡慧中 ; 柏佳宁 ; 张静 ; 何宏轩 ; 段明星 ; HU Hui-zhong ; BAI Jia-ning ; ZHANG Jing
	2010-06-10 ; 2010-06-10
关键词	热休克蛋白 基因克隆 原核表达 Heat shock protein Gene cloning Prokaryotic expression Q78
其他题名	Cloning and Expression of Human Heat Shock Protein 70 Gene and Identification of Expressed Product
中文摘要	目的克隆人热休克蛋白70(HSP70)基因,构建其原核高效表达载体。方法将PCR扩增的HSP70基因克隆到原核高效表达载体pET-28b中,转化大肠杆菌JM109。挑选阳性克隆,提取质粒pE28b70F,转化大肠杆菌BL21(DE3),IPTG诱导表达目标蛋白。结果在大肠杆菌中成功表达了HSP70,表达量约占细菌总蛋白的22·3%,其中可溶性目标蛋白占上清总蛋白的12%。Westernblot表明该目标蛋白具有与鼠抗人HSP70单抗特异结合的抗原活性。通过Ni2+-NTA亲和柱,从1L诱导产物中纯化出约1520mg重组蛋白,纯度在80%以上。结论已成功表达HSP70,为进一步研究其生物学功能和作用机制奠定了基础。; Objective To clone human heat shock protein 70(HSP70)gene for the construction of a prokaryotic expression vector.Methods Amplify HSP70 gene by PCR,clone into prokaryotic expression vector pET-28b,then transform to E.coli JM109.Screen the positive clones,extract plasmid pE28b70F and transform to E.coli BL21(DE3)for expression under induction of IPTG.Results The expressed HSP70 contained 22.3% of total somatic protein,of which 12% were soluble protein.Western blot showed specific reaction of the expressed protein with mouse anti-human HSP70 McAb.About 15-20 mg of recombinant protein was purified from 1 L of culture supernatant of recombinant E.coli by Ni~2+-NTA affinity column chromatography,and reached a purity of more than 80%.Conclusion HSP70 was successfully expressed,which laid a foundation of further study on its biological function and action mechanism.; 中国博士后科学基金(2003033120).
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/60586]
专题	清华大学
推荐引用方式 GB/T 7714	胡慧中,柏佳宁,张静,等. 人热休克蛋白70基因的克隆、表达及鉴定[J],2010, 2010.
APA	胡慧中.,柏佳宁.,张静.,何宏轩.,段明星.,...&ZHANG Jing.(2010).人热休克蛋白70基因的克隆、表达及鉴定..
MLA	胡慧中,et al."人热休克蛋白70基因的克隆、表达及鉴定".(2010).

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