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	异丙酚对脊髓兴奋性氨基酸含量的影响
	曾俊 ; 姚俊岩 ; 王泉云 ; 张艳丽 ; 翁浩 ; ZENG Jun ; YAO Junyan ; WANG Quanyun ; ZHANG Yanli ; WENG Hao
	2010-06-09 ; 2010-06-09
关键词	异丙酚 脊髓 缺血再灌注损伤 兴奋性氨基酸 Propofol Spinal cord Ischemia/reperfusion induced injury Excitatory amino acid Rabbit R96
其他题名	EFFECT OF PROPOFOL ON SPINAL EXCITATORY AMINO ACID ACCUMULATION
中文摘要	目的观察异丙酚对兔缺血再灌注损伤脊髓的保护作用以及对兴奋性氨基酸(excitatory amino acid,EAA)的影响。方法健康新西兰大白兔60只,雌雄各半,体重2.0～2.5kg。采用左肾下腹主动脉阻断法建立脊髓缺血再灌注损伤模型,阻断开始即泵入灌注液6mL/kg(不足部分均以10脂肪乳补充),灌注速度12mL/(kg.h),30min后停止灌注,开放腹主动脉。根据灌注液的不同,随机分为生理盐水组(A组)、10脂肪乳组(B组)、30mg/kg异丙酚组(C组)、40mg/kg异丙酚组(D组)、50mg/kg异丙酚组(E组)及60mg/kg异丙酚组(F组),每组10只。分别记录麻醉清醒即刻、再灌注后6、24和48h兔神经行为学评分;于再灌注后48h取L4～6节段脊髓组织计数脊髓前角正常神经元;采用高效液相色谱法测定脊髓组织中EAA含量。结果C、D、E、F组各时间点神经行为学评分明显优于A、B组(P<0.05),E组评分最高(P<0.05),A、B组间差异无统计学意义(P>0.05)。C、D、E、F组脊髓前角正常神经元明显多于A、B组(P<0.05),且E组多于C、D、F组(P<0.05)。A、B、C、D、E、F组脊髓组织EAA含量均明显高于正常值,其中A、B组最高(P<0.05),但A、B组间差异无统计学意义(P>0.05);E组最低(P<0.05)。谷氨酸、天门冬氨酸含量均与脊髓前角正常神经元计数及再灌注后48h神经行为学评分成负相关,相关系数分别为—0.613、—0.536、—0.874及—0.813(P<0.01)。结论异丙酚能降低缺血再灌注脊髓组织中EAA含量,减轻脊髓缺血再灌注损伤。; Objective To investigate the protective e ect of propofol on ischemia/reperfusion induced spinal cord injury in rabbits and its in uence on excitatory amino acid (EAA). Methods Sixty New Zealand white rabbits weighing 2.0-2.5 kg, half males and half females, were selected. The infrarenal circumaortic clamping model was used. And 6 mL/kg di erent uids were continuously infused through a catheter into the aorta distal to the clamping site at a speed of 12 mL/(kg?h) during the 30 minutes ischemia period. According to the di erent infusing liquids, the rabbits were randomized into 6 groups (n=10 per group): group A, normal saline; group B, 10 intralipid; group C, propofol 30 mg/kg; group D, propofol 40 mg/kg; group E, propofol 50 mg/kg; group F, propofol 60 mg/kg. At 0, 6, 24, and 48 hours after reperfusion, neurologic outcomes were scored on a Tarlov scale system. At 48 hours after reperfusion, the number of normal neurons in the anterior spinal cord was counted, and concentration of EAA in the lumbar spinal cord was measured by high performance liquid chromatography. Results The neuroethological score was better in groups C, D, E and F than that of groups A and B (P < 0.05), the score of group E was the highest (P < 0.05), and there was no signi cant di erence between group A and group B (P > 0.05). The number of normal neurons in the anterior spinal cord of groups C, D, E and F was greater than that of groups A and B (P < 0.05), and group E was greater than groups C, D and F (P < 0.05). The concentration of EAA in groups A, B, C, D, E and F was greater than that of normal tissue, the group E was the lowest (P < 0.05), the groups A and B were the highest (P < 0.05), and there was no signi cant di erence between group A and group B (P > 0.05). Concentrations of glutamate and aspartic acid were negatively correlated to normal neuron numbers in the anterior spinal cord and neuroethological scores 48 hours after reperfusion, and the corresponding correlation coe cient was — 0.613, — 0.536, — 0.874 and — 0.813, respectively (P < 0.01). Conclusion Propofol can signi cantly inhibit the accumulation of EAA in spinal cord and provide a protective e ect against the ischemia/reperfusion injury induced spinal cord in rabbits.; 国家教育部高等学校博士学科点专项科研基金(B12003610087)~~
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/59218]
专题	清华大学
推荐引用方式 GB/T 7714	曾俊,姚俊岩,王泉云,等. 异丙酚对脊髓兴奋性氨基酸含量的影响[J],2010, 2010.
APA	曾俊.,姚俊岩.,王泉云.,张艳丽.,翁浩.,...&WENG Hao.(2010).异丙酚对脊髓兴奋性氨基酸含量的影响..
MLA	曾俊,et al."异丙酚对脊髓兴奋性氨基酸含量的影响".(2010).

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