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	全反式维甲酸对移植心脏血管增殖病变的影响
	张明奎 ; 吴清玉 ; 胡建国 ; ZHANG Ming-kui ; WU Qing-yu ; HU Jian-guo
	2010-06-09 ; 2010-06-09
关键词	心脏移植 冠状血管病变 增殖细胞核抗原 大鼠 Heart transplantation Coronary artery disease Proliferating cell nuclear antigen Rat R654.2
其他题名	Effect of All-trans Retinoic Acid on Proliferative Artery Disease after Heart Transplantation
中文摘要	目的探讨全反式维甲酸(all-trans retinoic acid,atRA)对移植心脏血管增殖病变的影响。方法将近交系健康雄性Wistar大鼠16只作供体,SD大鼠16只作受体,采用Ono法建立大鼠异位心脏移植模型,用完全随机设计将动物分为慢性排斥组和atRA治疗组,每组8只。慢性排斥组术后给予环孢菌素A10mg/(kg.d),皮下注射;atRA治疗组术后同法给予环孢霉素A,并给予atRA10mg/(kg.d)灌胃。移植60d后取移植心脏,行HE、Masson、VanGieson染色,分析移植心脏排斥反应、血管狭窄及心肌纤维化程度;免疫组织化学染色检测细胞增殖核抗原(PCNA)。结果慢性排斥组心肌纤维化面积明显大于atRA治疗组(63.99%±11.91%vs.34.68%±6.34%),两组比较差异有统计学意义(t=8.377,P=0.000);慢性排斥组移植心脏血管狭窄指数高于atRA治疗组(62.86%±17.18%vs.40.10%±8.20%),atRA治疗组血管狭窄明显减轻,两组比较差异有统计学意义(t=3.913,P=0.006);慢性排斥组PCNA阳性细胞明显高于atRA治疗组(60.17±17.74vs.33.96±8.65),两组比较差异有统计学意义(t=5.387,P=0.001),且PCNA阳性细胞率与血管狭窄指数呈正相关(r=0.854,P=0.007)。结论全反式维甲酸可以通过细胞增殖途径抑制移植心脏血管病变。; Objective To investigate the effect of all-trans retinoic acid(atRA) on proliferative artery disease after heart transplantation.Methods Heterotopic heart transplantation model was established by Ono model with 16 inbred healthy male Wistar rats as donors and 16 SD rats as recipients.The rats were divided into chronic rejection group and atRA-treated group by complete random design,and there were 8 rats in each group.Rats in chronic rejection group were given Cyclosporine A 10 mg/(kg·d) by subcutaneous injection after operation,and those in atRA-treated group were given Cyclosporine A 10 mg/(kg·d) in the same way and atRA 10mg/(kg·d) by gavage.The transplanted hearts of rats were taken out 60 days after the transplantation.HE stain,masson stain and Van Gieson were done to analyze the rejection of transplanted hearts,the degree of vascular stenosis and myocardial fibrosis respectively.Immunohistochemistry was used to test proliferating cell nuclear antigen(PCNA).Results The area of myocardial fibrosis in chronic rejection group was obviously larger than that in atRA-treated group(63.99%±11.91% vs.34.68%±6.34%),and there was significant difference between two groups(t=8.377,P=0.000).The index of vascular stenosis in chronic rejection group was higher than that in atRA-treated group(62.86±17.18 vs.40.10±8.20).Vascular stenosis in atRA-treated group alleviated significantly,and there was significant difference between two groups(t=3.913,P=0.006).The PCNA positive cells in chronic rejection group were obviously more than that in atRA-treated group(60.17±17.74 vs.33.96±8.65),and there was significant difference between two groups(t=5.387,P≤0.001).There was a positive correlation between the PCNA positive cell ratio and the index of vascular stenosis(r=0.854,P=0.007).Conclusion All-trans retinoic acid can inhibit vascular disease after heart transplantation by cell proliferative pathway.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/59104]
专题	清华大学
推荐引用方式 GB/T 7714	张明奎,吴清玉,胡建国,等. 全反式维甲酸对移植心脏血管增殖病变的影响[J],2010, 2010.
APA	张明奎,吴清玉,胡建国,ZHANG Ming-kui,WU Qing-yu,&HU Jian-guo.(2010).全反式维甲酸对移植心脏血管增殖病变的影响..
MLA	张明奎,et al."全反式维甲酸对移植心脏血管增殖病变的影响".(2010).

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