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	不同方法检测抗梅毒抗体阳性标本的分析
	白雪梅 ; 闪全忠 ; 刘欧 ; 阮芳 ; 祁小真 ; BAI Xuemei ; SHAN Quanzhong ; LIU Ou ; RUAN Fang ; QI Xiaozhen
	2010-06-09 ; 2010-06-09
关键词	梅毒螺旋体 免疫印迹法 TPHA Treponema pallidum western blot TPHA R446.6
其他题名	Evaluation of different methods for detection of Treponema pallidum antibody-positive samples
中文摘要	目的对TPHA检测血清抗梅毒螺旋体抗体阳性的标本,同时用另外3种方法进行检测,以探讨阳性结果是否存在方法学导致的假阳性。方法3957例无梅毒症状的普通病人为实验组,344例性病门诊病人为对照组。用TPHA进行抗梅毒抗体筛查,检测阳性的标本再用酶免疫法、甲苯胺红不加热血清试验(TRUST)、免疫印迹法进行检测。以免疫印迹法为标准对检测结果进行对比分析。结果实验组中检出TPHA阳性60例,经免疫印迹法检测确认阳性57例,临界2例,1例为假阳性,酶免疫法阳性53例,TRUST阳性23例。对照组中TPHA阳性40例,免疫印迹法确认阳性40例,酶免疫法阳性40例,TRUST阳性32例。结论TPHA、EIA测定抗梅毒抗体有较高的阳性符合率,2种方法检测抗体为阳性的患者,几乎全部存在既往感染或隐性感染。对TRUST的结果则应综合分析。; Objective To analyze the false-positive results of Treponema pallidum antibody caused by 3 different assay in comparison with Treponema pallidum hemagglutination assay (TPHA).Methods Research group included 3957 clinically asymptomatic syphilis patients,and control group was 344 outpatients with sex-transmitted diseases (STD).The serum samples from the patients who were TPHA-positive were tested in parallel by enzymeimmunoassay (EIA) and syphilis toluidine red untreated serum test (TRUST).Western blot (WB) was performed as confirmatory test.Results In the clinically asymptomatic patients,60 were TPHA-positive.Among them 57 were confirmed by western blot assay,and 1 was false-positive and 2 were borderline in WB.Of the 60 TPHA-positive patients,53 were positive in EIA and 23 were positive in TRUST.In STD patients 40 were TPHA,WB and EIA-positive but 32 were TRUST-positive.Conclusions The results of TPHA and EIA were consistent for diagnosis of syphilis patients who may suffer from previous or latent infection.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/58833]
专题	清华大学
推荐引用方式 GB/T 7714	白雪梅,闪全忠,刘欧,等. 不同方法检测抗梅毒抗体阳性标本的分析[J],2010, 2010.
APA	白雪梅.,闪全忠.,刘欧.,阮芳.,祁小真.,...&QI Xiaozhen.(2010).不同方法检测抗梅毒抗体阳性标本的分析..
MLA	白雪梅,et al."不同方法检测抗梅毒抗体阳性标本的分析".(2010).

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