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	人类羊膜细胞表达神经干细胞特异性蛋白研究
	蔡哲 ; 潘琳 ; 舒峻 ; 张岚 ; 郭艳茹 ; 耿同超 ; 牟亮 ; 左萍萍 ; CAI Zhe ; PAN Lin ; SHU Jun
	2010-06-09 ; 2010-06-09
关键词	人类羊膜细胞 神经前体细胞 巢蛋白 musashi-1 human amnion cell neural progenitor cell nestin musashi-1 R741
其他题名	Specific proteins of neural stem cell expressed by human amnion cells
中文摘要	目的利用神经干细胞特异性标志蛋白鉴定人类羊膜组织中多分化潜能神经前体细胞的存在。方法利用免疫组织化学法检测人类羊膜组织和培养人类羊膜细胞中巢蛋白(nestin)、胶质原纤维酸性蛋白(GFAP)、musashi-1、波形蛋白(vi mentin)和PSA-NCAM等神经干细胞特异性标志蛋白的表达。结果人羊膜组织中存在nestin/GFAP双阳性细胞,此外,还表达musashi-1、vi mentin和PSA-NCAM等神经干细胞特异性标志蛋白;培养羊膜细胞中存在vi mentin和PSA-NCAM阳性细胞,以及nestin/GFAP双阳性细胞。结论羊膜组织和培养羊膜细胞中有神经干细胞特异性标记蛋白的表达。; ObjectiveTo characterize the neural progenitor cell in the human amnion mesenchyme and epithelial layer with specific mark proteins of neural stem cell.MethodsExpressions of specific mark proteins of neural stem cell including nestin, glial fibrillary acidic protein (GFAP), musashi-1, vimentin and PSA-NCAM in human amnion tissue and cultured amniotic cells were determined by immunohistochemistry and immunofluorescence staining.ResultsExpressions of pluripotent neural stem cell specific makers (nestin, musashi-1, vimentin and PSA-NCAM) were detected in the human amnion mesenchyme and epithelial layer. In addition, cultured amniotic cells were expressed several neural stem cell specific markers including nestin, GFAP and PSA-NCAM. Nestin+ and GFAP+ double positive cells were identified in the human amnion tissue and cultured amniotic cells by immunohistochemistry and immunofluorescence staining.ConclusionSpecific mark proteins of neural stem cell are expressed in human amnion tissue and cultured amniotic cells.; 北京市自然科学基金重点项目(No.5041002); 国家自然科学基金项目(No.30471773)。
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/58633]
专题	清华大学
推荐引用方式 GB/T 7714	蔡哲,潘琳,舒峻,等. 人类羊膜细胞表达神经干细胞特异性蛋白研究[J],2010, 2010.
APA	蔡哲.,潘琳.,舒峻.,张岚.,郭艳茹.,...&SHU Jun.(2010).人类羊膜细胞表达神经干细胞特异性蛋白研究..
MLA	蔡哲,et al."人类羊膜细胞表达神经干细胞特异性蛋白研究".(2010).

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